Serological and Molecular Characterisation of Cucumber mosaic virus Infecting Lagenaria siceraria L. in Adim, Biase L.G.A., Cross River State, Nigeria
O. I. Eyong, A. T. Owolabi, A. A. J. Mofunanya, E. E. Ekpiken
Annual Research & Review in Biology · pp. 45–52 · Published 2 Jun 2020
10.9734/arrb/2020/v35i430211Abstract
Cucumber mosaic virus (CMV) is one of the most important viral pathogens infecting a wide range of plant species in Nigeria. Mosaic and mottle symptoms were observed on Lagenaria siceraria L. in Adim Southern-Nigeria in 2018 and the infected leaves collected for investigation. This research was aimed at characterising the virus responsible for this infection with a view to identifying it. Antigen coated plate (ACP) enzyme linked-immunosorbent assay (ELISA) and gene sequencing were employed methods in the characterisation process. The Amplified Complementary Deoxyribonucleic Acid (cDNA) was cloned and the nucleotide sequence was determined. Result of serology revealed that the virus belonged to the genus Cucumovirus while the gene sequence obtained when compared to known virus sequences present in the GenBank using the Basic Local Alignment Search Tool (BLAST) program available at National Centre for Biotechnology Information (NCBI) revealed 97% sequence homologue with Cucumber mosaic virus confirming it as Cucumber mosaic virus. This is the first report of CMV infecting L. siceraria in Nigeria. I recommend further studies on insect and host range test of this virus be carried on.
References (26)
- 1 The Sweetpotato or Silverleaf Whiteflies: Biotypes of Bemisia tabaci or a Species Complex? [DOI]
- 2 Arthropod Transmission of Plant Viruses: a New Synthesis [DOI]
- 3 INVASION BIOLOGY OF THRIPS [DOI]
- 4 The melon fruit fly, Bactrocera cucurbitae: A review of its biology and management [DOI]
- 5 A review of relationships between interspecific competition and invasions in fruit flies (Diptera: Tephritidae) [DOI]
- 6 Optimization of diagnostic RT-PCR protocols and sampling procedures for the reliable and cost-effective detection of Cassava brown streak virus [DOI]
- 7 Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus [DOI]
- 8 The melon fruit fly, Bactrocera cucurbitae: A review of its biology and management [DOI]
- 9 Monoclonal antibody-based serological assays and immunocapture-RT-PCR for detecting Rice dwarf virus in field rice plants and leafhopper vectors [DOI]
- 10 Breeding for Disease Resistance in Cucurbits [DOI]
- 11 Biological characterization and complete nucleotide sequence of a Tunisian isolate of Moroccan watermelon mosaic virus [DOI]
- 12 Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm [DOI]
- 13 Biological and Molecular Characterization of Melon‐Infecting Kyuri Green Mottle Mosaic Virus in Indonesia [DOI]
- 14 Development and detection application of monoclonal antibodies against Zucchini yellow mosaic virus [DOI]
- 15 The Use of 3' Non-coding Nucleotide Sequences in the Taxonomy of Potyviruses: Application to Watermelon Mosaic Virus 2 and Soybean Mosaic Virus-N [DOI]
- 16 Biological and Molecular Characterization of Potato Infecting Alfalfa Mosaic Virus in Egypt [DOI]
- 17 Characterization of an emerging isolate of watermelon mosaic virus in Turkey
- 18 The Impact of Intercropping Squash with Non-Crop Vegetation Borders on the Above-Ground Arthropod Community [DOI]
- 19 Dot-immunobinding assay for zucchini yellow mosaic virus using polyclonal and monoclonal antibodies. [DOI]
- 20 Viruses in weeds in Dioscorea yam fields in Nigeria
- 21 Zucchini yellow mosaic virus [DOI]
- 22 An Isolate of Cucumber Mosaic Virus from Fluted Pumpkin in Nigeria [DOI]
- 23 Characterization and Identification of a Potyvirus Causing Mosaic Disease of Cucurbita moschata Duch Ex. Poir in Calabar, South East Nigeria [DOI]
- 24 Detection of Potyvirus Using RT-PCR and ACP-ELISA of Dioscorea Species and In Vitro Shoot Multiplication of the Virus Free Plants
- 25 A strain of papaya ringspot virus naturally infecting cucumber (Cucumis sativus L.) in Calabar, South Eastern Nigeria
- 26 Virus taxonomy, 6th report of the International Committee on Taxonomy of Viruses.
Cited by 2
Ramasamy Selvarajan, V. Balasubramanian, M. Mahalakshmi · Plant RNA Viruses · 2023
Showing 1 of 2 known citations — external sources report more than can currently be individually listed.
Related research
- Epidemiological Study of Influenza A(H1N1) Virus Infection during Lockdown Due to COVID-19 Pandemics in Uttarakhand — shares topic coverage
- Innovative and Rapid Detection Methods for SARS-CoV-2 Infections: Recent Developments Worldwide — shares topic coverage
- A Comparative Study between Covid-19 Positive versus Suspected Patients: A Single-Center Study in Bangladesh — shares topic coverage
- Major Symptoms and Outcome of Covid-19 Positive Patients: A Study in a Tertiary Care Hospital in Bangladesh — shares topic coverage
- Molecular Detection of Astrovirus in Diarrhoeic Stools of Children in North East Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
51
Page views
0
PDF downloads
7
Outbound clicks
2
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.