Skip to content
Research Article Open access CC BY 4.0

Innovative and Rapid Detection Methods for SARS-CoV-2 Infections: Recent Developments Worldwide

Aratrika Chakraborty, Atin Adhikari

Asian Journal of Medicine and Health · pp. 181–194 · Published 11 Aug 2022

10.9734/ajmah/2022/v20i1030518

Abstract

There are no effective treatments for COVID-19 caused by SARS-CoV-2 viruses worldwide and the effects of vaccines on new genetic variants of the virus are still uncertain. Therefore, initial diagnosis and management are essential for controlling the pandemic. The conventional diagnosis methods for SARS-CoV-2 include molecular tests, serology tests, and computed tomography (CT). However, a few innovative, rapid, and promising detection methods were developed in the last year and this year in different countries of the world. We have discussed the advantages and challenges of these new diagnostic methods besides discussing the widely adopted conventional approaches mentioned above. We have summarized the most recent and conventional diagnosis methods, including commercially available devices and kits according to their SARS-CoV-2 detection accuracy rates and completion times. We have also discussed the ongoing research in this field. Finally, we have identified some thrust areas of research that could help to identify the new variants of SARS-CoV-2 rapidly and accurately. Our review findings indicate that CRISPR-based FELUDA, SHERLOCK, Hyris kit, and COVIRAP are low time consuming, user friendly, and very appropriate for low-resource countries. Although several diagnostic methods are available, we need more efficient diagnostic tools for SARS-CoV-2 with respect to cost efficiency, accuracy, rapid detection, and user-friendliness, especially for new variants in remote areas of the world.

COVID-19 SARS-CoV-2 RT-PCR CT scan diagnostics rapid detection method serology

References (49)

  1. 1 CRISPR–Cas12-based detection of SARS-CoV-2 [DOI]
  2. 2 Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures [DOI]
  3. 3 Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing [DOI]
  4. 4 Artificial intelligence for the detection of COVID-19 pneumonia on chest CT using multinational datasets [DOI]
  5. 5 Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza [DOI]
  6. 6 Chest CT in COVID-19: What the Radiologist Needs to Know [DOI]
  7. 7 Rapid isothermal amplification and portable detection system for SARS-CoV-2 [DOI]
  8. 8 Testing at scale during the COVID-19 pandemic [DOI]
  9. 9 Diagnostics for SARS-CoV-2 detection: A comprehensive review of the FDA-EUA COVID-19 testing landscape [DOI]
  10. 10 Aptasensors for detection of microbial and viral pathogens [DOI]
  11. 11 Next generation sequencing of SARS-CoV-2 genomes: challenges, applications and opportunities [DOI]
  12. 12 Mini review: Recent progress in RT-LAMP enabled COVID-19 detection [DOI]
  13. 13 A DNA Aptamer Based Method for Detection of SARS-CoV-2 Nucleocapsid Protein [DOI]
  14. 14 Laboratory Testing Methods for Novel Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) [DOI]
  15. 15 Nucleocapsid Protein as Early Diagnostic Marker for SARS [DOI]
  16. 16 SARS-CoV-2 detection in different respiratory sites: A systematic review and meta-analysis [DOI]
  17. 17 Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP) [DOI]
  18. 18 CRISPR Systems for COVID-19 Diagnosis [DOI]
  19. 19 Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology [DOI]
  20. 20 Colorimetric RT-LAMP SARS-CoV-2 diagnostic sensitivity relies on color interpretation and viral load [DOI]
  21. 21 A serological aptamer-assisted proximity ligation assay for COVID-19 diagnosis and seeking neutralizing aptamers [DOI]
  22. 22 Identification of SARS-CoV-2-against aptamer with high neutralization activity by blocking the RBD domain of spike protein 1 [DOI]
  23. 23 Clinical diagnostic value of CT imaging in COVID-19 with multiple negative RT-PCR testing [DOI]
  24. 24 Severity assessment of COVID-19 using CT image features and laboratory indices [DOI]
  25. 25 Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum: Comparison with results of other viral markers [DOI]
  26. 26 Real-life clinical sensitivity of SARS-CoV-2 RT-PCR test in symptomatic patients [DOI]
  27. 27 Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection [DOI]
  28. 28 CRISPR-Cas System: An Approach With Potentials for COVID-19 Diagnosis and Therapeutics [DOI]
  29. 29 Rapid molecular diagnostics of COVID-19 by RT-LAMP in a centrifugal polystyrene-toner based microdevice with end-point visual detection [DOI]
  30. 30 Next-Generation Sequencing Reveals the Progression of COVID-19 [DOI]
  31. 31 The Importance of Chest CT Scan in COVID-19.
  32. 32 Aptamers, the bivalent agents as probes and therapies for coronavirus infections: A systematic review [DOI]
  33. 33 Emerging diagnostic tools for detection of COVID-19 and perspective [DOI]
  34. 34 Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics [DOI]
  35. 35 CRISPR/Cas System: A Potential Technology for the Prevention and Control of COVID-19 and Emerging Infectious Diseases [DOI]
  36. 36 COVID-19 Pandemisinde SARS-CoV-2 ve Mikrobiyolojik Tanı Dinamikleri [DOI]
  37. 37 CRISPR/Cas as a Potential Diagnosis Technique for COVID-19.
  38. 38 Positive Chest CT Features in Patients With COVID-19 Pneumonia and Negative Real-Time Polymerase Chain Reaction Test [DOI]
  39. 39 Fast SARS-CoV-2 virus detection using disposable cartridge strips and a semiconductor-based biosensor platform [DOI]
  40. 40 Can chest CT improve sensitivity of COVID-19 diagnosis in comparison to PCR? A meta-analysis study [DOI]
  41. 41 The role of CT imaging for management of COVID-19 in epidemic area: early experience from a University Hospital [DOI]
  42. 42 Selection of high affinity RNA ligands to the bacteriophage R17 coat protein [DOI]
  43. 43 The Role of Repeat Chest CT Scan in the COVID-19 Pandemic [DOI]
  44. 44 CRISPR/Cas-New Molecular Scissors in Diagnostics and Therapeutics of COVID-19 [DOI]
  45. 45 CRISPR vs COVID-19: How can Gene Editing Help Beat a Virus? [DOI]
  46. 46 Accuracy of Low-Dose Chest CT Scan in Detection of COVID-19 [DOI]
  47. 47 Evaluation of the diagnostic accuracy of a new point-of-care rapid test for SARS-CoV-2 virus detection [DOI]
  48. 48 Assay Techniques and Test Development for COVID-19 Diagnosis [DOI]
  49. 49 LAMP-Seq: Population-Scale COVID-19 Diagnostics Using Combinatorial Barcoding [DOI]

Cited by 0

No indexed citations yet.

Article metrics

Real usage data collected on this platform.

58

Page views

0

PDF downloads

18

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.