Skip to content
Research Article Open access CC BY 4.0

Screening and Media Optimization for Enhancing L-asparaginase Production, an Anticancer Agent, from Different Filamentous Fungi in Solid State Fermentation

Ali Mohamed Elshafei, Dina Helmy El-Ghonemy

Biotechnology Journal International · pp. 1–15 · Published 2 Sep 2015

10.9734/BBJ/2015/19728

Abstract

Aim: The aim of present study was to screen new potent fungal isolates and microorganisms possessing extracellular L-asparaginase production capacity. In addition, optimization of cultural and environmental conditions required for enzyme production will be carried out for the highest L-asparaginase producer in solid state fermentation (SSF) technique using agro-industrial residues. Study Design: Screening and physiological studies on the formation of L-asparaginase by Trichoderma viride F2 in order to obtain the optimum cultural and environmental conditions required for enzyme production. Place and Duration of Study: Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Cairo, Egypt, between July 2013 and June 2015. Methodology: Optimization of physical and nutritional parameters for enzyme production was investigated. Various locally available agro-industrial residues have been screened individually or as mixtures for L-asparaginase production. The combination of Rice husk (RH) with wheat bran (WB) (3:2) proved to be an efficient mixture for enzyme production as it gave the highest enzyme activity (71.87±3.19 U/g-ds) when compared to individual RH (66.71±2.76 U/g-ds) or WB (62.28±2.13 U/g-ds) substrates. Results: Maximal L-asparaginase production (113.43±5.11 U/g-ds) by T. viride F2 was obtained with moisture content of 75%, an inoculums size of 1 x 108 spores/ml and an initial medium pH of 5.0 when incubated at 28ºC for four days. Presence of Tween 20 enhanced enzyme production by 1.19 folds. Glucose (1.0%), Casein (1.5%) and MgCl2 (0.05%) were found to be the best carbon, organic nitrogen and ion sources, respectively. Supplementation of the medium with NaNO3 (0.15%) as an inorganic nitrogen source further increased L-asparaginase production. Under these optimized conditions, L-asparaginase production by T. viride F2 was maximum with a yield of 276.5±13.4 U/g-ds in SSF, which was more than 19-fold enhancement in enzyme activity as compared to that obtained in the basal medium (SmF) (14.23±0.87 U/ml). Conclusion: The results suggest that choosing a suitable substrate coupled with optimization of different parameters can improves enzyme production markedly. Moreover, the production of L-asparaginase from a process based on RH and WB as substrates in SSF is economically attractive due to abundant substrates availability in agriculture-based countries with cheaper cost.

L-asparaginase filamentous fungi optimization screening solid state fermentation

References (40)

  1. 1 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding [DOI]
  2. 2 New developments in solid state fermentation: I-bioprocesses and products [DOI]
  3. 3 Asparaginase and Glutaminase Activities of Micro-organisms [DOI]
  4. 4 A rapid plate assay for screening l ‐asparaginase producing micro‐organisms [DOI]
  5. 5 Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation [DOI]
  6. 6 Production of L-asparaginase by filamentous fungi [DOI]
  7. 7 Thermostable and alkaline-tolerant microbial cellulase-free xylanases produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review [DOI]
  8. 8 Production, purification and characterization of L-asparaginase from Streptomyces gulbargensis [DOI]
  9. 9 L-asparaginase production by Streptomyces albidoflavus [DOI]
  10. 10 Purification and characterization of glutaminase-free l-asparaginase from Pectobacterium carotovorum MTCC 1428 [DOI]
  11. 11 Production of cellulase-free endoxylanase from novel alkalophilic thermotolerent Bacillus pumilus by solid-state fermentation and its application in wastepaper recycling [DOI]
  12. 12 Production of L-Asparaginase, an Anticancer Agent, From Aspergillus niger Using Agricultural Waste in Solid State Fermentation [DOI]
  13. 13 ISOLATION, MOLECULAR IDENTIFICATION AND OPTIMIZATION OF FERMENTATION PARAMETERS FOR THE PRODUCTION OF L-ASPARAGINASE, AN ANTICANCER AGENT BY FUSARIUM EQUISETI [DOI]
  14. 14 Hydrolysis of wheat bran and straw by an endoxylanase: production and structural characterization of cinnamoyl-oligosaccharides [DOI]
  15. 15 Enhancement of L-Asparaginase Production by Isolated Bacillus circulans (MTCC 8574) Using Response Surface Methodology [DOI]
  16. 16 Studies on nutritional and oxygen requirements for production of L-asparaginase by Enterobacter aerogenes [DOI]
  17. 17 Mixed substrate solid state fermentation for production and extraction of lipase from Aspergillus niger MTCC 2594 [DOI]
  18. 18 L-asparaginase from marine derived fungal endophytes of seaweeds
  19. 19 Production of Tumor-Inhibitory L-Asparaginase by Submerged Growth of Serratia marcescens [DOI]
  20. 20 Design of experiments and artificial neural network linked genetic algorithm for modeling and optimization of L-asparaginase production by Aspergillus terreus MTCC 1782 [DOI]
  21. 21 Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization [DOI]
  22. 22 Screening and Isolation of Novel Glutaminase Free L-asparaginase from Fungal Endophytes [DOI]
  23. 23 L-ASPARAGINASE-AN ANTI TUMOR AGENT PRODUCTION BY FUSARIUM EQUISETI USING SOLID STATE FERMENTATION [DOI]
  24. 24 L-Asparaginase production by actinomycetes isolated from some Thai medicinal plant rhizosphere soils
  25. 25 Asparaginase production by endophytic fungi from Thai medicinal plants: cytoxicity properties.
  26. 26 Production of β-N-acetylhexosaminidase of Verticillium lecanii by solid state and submerged fermentations utilizing shrimp waste silage as substrate and inducer [DOI]
  27. 27 Some studies on optimization of extraction process for protease production in SSF [DOI]
  28. 28 Studies on Optimization of Growth Parameters for L-Asparaginase Production byStreptomyces ginsengisoli [DOI]
  29. 29 Improved xylanase production from a haloalkalophilic Staphylococcus sp. SG-13 using inexpensive agricultural residues [DOI]
  30. 30 Isolation, identification and screening of potential xylanolytic enzyme from litter degrading fungi [DOI]
  31. 31 Physical and nutritional factors affecting the production of amylase from species of bacillus isolated from spoiled food waste [DOI]
  32. 32 Production of L-asparaginase by Serratia marcescens SB08: Optimization by response surface methodology
  33. 33 Impact of Carbon and Nitrogen Sources on L-Asparaginase Production by Isolated Bacillus circulans (MTCC 8574): Application of Saturated Plackett-Burman Design
  34. 34 Screening, Production and Optimization of L-Asparaginase From Soil Bacteria Isolated in Ibadan, South-western Nigeria [DOI]
  35. 35 Production of L-asparaginase from Natural Substrates by Aspergillus terreus MTCC 1782: Optimization of Carbon Source and Operating Conditions [DOI]
  36. 36 Application of Statistical Experimental Designs for the Optimization of Medium Constituents for the Production of L-Asparaginase by Serratia Marcescens [DOI]
  37. 37 High Efficiency, Selectivity Against Cancer Cell Line Of Purified L-Asparaginase From Pathogenic Escherichia Coli [DOI]
  38. 38 Production and optimization of L-asparaginase by Bacillus sp. KK2S4 from corn cob [DOI]
  39. 39 L-asparaginase activity in Aeromonas sp. isolated from freshwater mussel.
  40. 40 L‐Asparaginase: A Review [DOI]

Cited by 35

Applications and Scope of Microbial L-Asparaginase

Rachna Shashikant Jadhav, Himanshu Sharma, Saurabh Kulshrestha · Pharmaceutically Relevant Microbial Enzymes · 2026

Optimization of the fermentation process for fructosyltransferase production by Aspergillus niger FS054

Yingzi Wu, Yuewen Zhang, Xiaoyu Zhong · Microbial Cell Factories · 2025

Characterization of L-asparaginase from marine-derived Aspergillus niger AKV-MKBU, its antiproliferative activity and bench scale production using industrial waste

Anjana K. Vala, Bhumi Sachaniya, Dushyant Dudhagara · International Journal of Biological Macromolecules · 2018

Therapeutic impact of purified Trichoderma viride L-asparaginase in murine model of liver cancer and in vitro Hep-G2 cell line

Dina H. El-Ghonemy, Sanaa A. Ali, Rehab M. Abdel-Megeed · Journal of Genetic Engineering and Biotechnology · 2023

L-asparaginase from Aspergillus oryzae spp.: effects of production process and biochemical parameters

Marília Crivelari da Cunha, Jessika Gonçalves dos Santos Aguilar, Santa Maria Del Rosário Orrillo Lindo · Preparative Biochemistry & Biotechnology · 2021

Microbial fermentation biotechnology of cooked chicken bone novel substrate for l -asparaginase production

Essam A. Makky, Muna Jalal Ali · Karbala International Journal of Modern Science · 2017

Potential of Anti-Cancer Activity of Secondary Metabolic Products from Marine Fungi

Efaq Noman, Muhanna Mohammed Al-Shaibani, Muhammed Adnan Bakhrebah · Journal of Fungi · 2021

Biological investigations of Aspergillus ficuum via in vivo, in vitro and in silico analyses

Zafar Ali Shah, Khalid Khan, Tanzeel Shah · Scientific Reports · 2023

Optimizing the culture conditions for L-Asparaginase production from endophytic fungus Curvularia sp. LCJ413 through conventional and statistical approach

Anbarasu Kathiravan, Elangovan Udayan, John Joel Gnanadoss · Journal of Experimental Biology and Agricultural Sciences · 2023

Showing 11 of 35 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

88

Page views

0

PDF downloads

45

Outbound clicks

35

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.