Antibiotic Susceptibility Profile of Bacteria Isolated from Fitness Machines in Selected Fitness Centers at Akure and Elizade University in Ondo State Nigeria

Abstract

Aim: This study seeks to determine the antibiotic susceptibility pattern of bacteria isolated from surfaces of fitness machines at fitness center located at Elizade University and Akure town. Methods: Samples were collected from the different site of gym equipment including thread mill (handle, floor), bicep bench (handle), bike (handle, paddle), cruncher (handle, elbow) using sterile swab stick moistened with sterile buffered physiological solution. The swab sticks were immediately transferred to the laboratory for analysis. Standard microbiological techniques were used to identify the bacterial isolates. The antibiotic susceptibility profile of the isolates was determined by using standard antibiotics discs.  Results: Out of the 31 isolates identified, Staphylococcus aureus 12(38.7%) was the predominant bacteria followed by Bacillus spp. 11(35.5%), Klebsiella spp. 4(12.9%), E. coli and Staphylococcus saprophyticus 2(6.45%) and Enterococcus spp. 1(3.23%). The susceptibility profile showed that all isolates were resistant to Amoxicillin (AM) and Augmentin (AU), Staphylococcus spp. isolated from different surfaces shows different susceptibility pattern to the used antibiotics, while Bacillus spp. Klebsiella spp. and E. coli also confer resistance to more than one commonly used antibiotic. Conclusion: The results showed the occurrence of potential pathogenic bacteria in which their presence on the equipment surfaces could easily be transmitted between users and to the environment generally. The spread of these potential pathogenic microorganisms in the fitness centre can be prevented through frequent hand washing and use of hand sanitizer as well as daily cleaning of equipment surfaces before and after activities with disinfectants.