Accurate Estimation of Some Pharmaceutical Compounds Using HPLC Technology

Abstract

For the assessment of different chemical components in pharmaceutical and biological samples, High Performance Liquid Chromatography (HPLC) can be defined as a commonly utilized analytical method in pharmaceutical samples. HPLC offers high sensitivity, specificity, and accuracy, making it a preferred method for the analysis of complex samples. In this technique, the sample is prepared by extracting, purifying, and concentrating the target analyte from the matrix. Mobile and stationary phases are after that used in chromatographic separation of the prepared sample. Several detectors, including fluorescence, UV-vis, and mass spectrometry, are used to identify and measure the eluted compounds. HPLC is capable of estimating a wide range of elements, including small molecules, peptides, and proteins, making it an essential tool in pharmaceutical research and development. The theoretical plates, peak tailing, and % assay have not been significantly impacted by the deliberate alterations made to the method. This implies that the existing technique is robust. The low values of LOQ and LOD show how sensitive the suggested approach is. The current research used a standard drug solution with six replicates to examine the system suitability parameters. It was discovered that the computed parameters fell under the acceptable criteria. The theoretical plate count, tailing factor, and HETP parameters are all within acceptable ranges. The results of this work are compared with those of earlier research. Upon analyzing the chart, it can be inferred that the proposed method presents certain benefits. The investigation employed a Kromasil C18 column. The obtained retention time is comparatively lower than that of all other methodologies. The maximum quantifiable threshold is 600 μg/mL, surpassing or equating all of the aforementioned techniques. The duration of the execution is also minimal. Therefore, the author draws the conclusion that the HPLC method proposed exhibits sensitivity and reproducibility in analysis of etodolac and thiocolchicoside in combined tablet dosage form, while also requiring a short analysis time.