Resistant Gene Analogue Marker(S) Screening against Yellow Mosaic Disease in Mungbean [Vigna radiata (L.) Wilczek]
Rishee K. Kalaria, Jigar Bhabhor, K. G. Modha
International Journal of Plant & Soil Science · pp. 261–279 · Published 30 Dec 2023
10.9734/ijpss/2023/v35i244317Abstract
During Rabi 2021–2022, 100 genotypes of mungbean were tested for resistance to yellow mosaic disease in the field using the disease grading system. Out of the 100 mungbean genotypes, 49 were resistant to the yellow mosaic disease, 22 as Moderately resistant, 11 as Moderately susceptible, 3 as susceptible, and 15 were highly susceptible. The sequenced virus shares 98.94% similarity with the whole coding sequence of the MYMIV-Mb02 coat protein (AV1) gene from the mungbean yellow mosaic India virus strain, accession number GQ387502.1., as per the results of the BLAST program. As a result, the variation was designated mungbean yellow mosaic India virus isolates NAU-RJ coat protein gene segment and assigned the accession number ON622515.1. The current study examined Six resistant (40 C, NMS-21-01, NMS-21-06, NMS-21-22, NMS-21-49, NMS-21-95) and six highly susceptible (GM 4, NMS-21-23, NMS-21-24, NMS-21-40, NMS-21-68, NMS-21-69). In order to identify the six susceptible and six resistant mungbean genotypes, nine pairs of RGA primers were employed. Five pairs of RGA primers were successfully amplified in all resistant genotypes as a consequence, but not in all highly susceptible genotypes. The results of this study suggest that five pairs of RGA markers can effectively distinguish between the genotypes of mungbean that are highly susceptible and resistant. The long-lasting YMV resistance of these RGA markers makes them useful for mapping resistance genes and marker validation studies.
References (16)
- 1 Molecular cloning: A laboratory manual [DOI]
- 2 MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0 [DOI]
- 3 Molecular plant-microbe interactions [DOI]
- 4 Geminivirus strain demarcation and nomenclature [DOI]
- 5 Genome sequence of mungbean and insights into evolution within Vigna species [DOI]
- 6 A source of resistance against yellow mosaic disease in soybeans correlates with a novel mutation in a resistance gene [DOI]
- 7 Multiple disease resistance in mungbean with special emphasis on mungbean yellow mosaic virus.
- 8 Screening Mungbean [Vigna radiata (L.) Wilczek] Genotypes for Mungbean Yellow Mosaic Virus Resistance under Natural Condition [DOI]
- 9 Molecular Detection and Characterization of Coat Protein Gene of Mungbean Yellow Mosaic Virus (MYMV) from Karnataka [DOI]
- 10 Resistant Gene Analogous Marker Assisted Selection of Yellow Mosaic Virus Resistant Genotypes in Greengram (Vigna radiata) [DOI]
- 11 Coat protein based characterization of Mungbean yellow mosaic virus in Tamil Nadu
- 12 Screening of Resistance Gene Analogue Marker(S) in Indian Bean [Lablab purpureus (L.) Sweet] against Yellow Mosaic Disease [DOI]
- 13 Journal of Pharmacognosy and Phytochemistry [DOI]
- 14 International Journal of Current Microbiology and Applied Sciences [DOI]
- 15 JOURNAL OF MYCOLOGY AND PLANT PATHOLOGY [DOI]
- 16 Identification and Characterization of Mungbean Yellow Mosaic Virus (MYMV) Resistance in Vigna Species
Cited by 2
M. Shilpa, B. Manjunath, C.M. Sunil, Sudarshan, G.K., Nagaraju, N · Journal of Advances in Biology & Biotechnology · 2024
Barsha Priyadarshini Das, Jyoti Prakash Sahoo, Manaswini Mahapatra · Plant Molecular Biology Reporter · 2025
Related research
- Biochemical and Molecular Characterization of Food Contaminating Bacteria Isolates from Food Stall Vegetables — shares topic coverage
- Identification, Characterization and Purification of MSC_0265, a Potential Immunogenic Antigen Homologue of Mycoplasma mycoides subsp. mycoides in Mycoplasma capricolum subsp. capripneumoniae — shares topic coverage
- Molecular Characterization and in-vitro Regeneration of Wild Ganoderma lucidum from Abuja, Nigeria — shares topic coverage
- Isolation and Identification of Resistant Bacteria from Petroleum Producing Vicinity by Gene Amplification and Sequencing — shares topic coverage
- Computational Insight into PCR Amplified Pectate Lyase Genes from Different Species of Aspergillus — shares topic coverage
Article metrics
Real usage data collected on this platform.
22
Page views
0
PDF downloads
0
Outbound clicks
2
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.