Regulatory Role of DNA Methylation and Its Significance in Plants
Rao Saad Rehman, Mujahid Ali, Syed Ali Zafar, Hassan Bashir, Asad Nadeem Pasha, Abdullah Javed, Naveed Ali Ashraf, Waqar Ali Shah
Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 1–15 · Published 1 Jul 2022
10.9734/ajbgmb/2022/v11i330267Abstract
DNA methylation is a well-known epigenetic modification that is essential for gene regulation and genome stability. Anomalies in plant development can result from aberrant DNA methylation patterns. DNA methylation is much more important in plants with more complicated genomes when it comes to growth and abiotic stress tolerance. Dynamic regulation via de novo methylation, maintenance of methylation, and active demethylation, which are catalysed by diverse enzymes that are targeted by different regulatory mechanisms, results in a unique DNA methylation state. We explain DNA methylation in plants, including methylating and demethylating enzymes and regulatory changes, as well as the coordination of methylation and demethylation activities by a mechanism known as the methylstat. We also explain the roles of DNA methylation in regulating transposon silencing, gene expression, and chromosome interactions, as well as the intervention of DNA methylation in plant responses to biotic and abiotic stresses.
References (72)
- 1 High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects [DOI]
- 2 Rationally engineered Cas9 nucleases with improved specificity [DOI]
- 3 Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew [DOI]
- 4 Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements [DOI]
- 5 DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins [DOI]
- 6 Enhanced proofreading governs CRISPR–Cas9 targeting accuracy [DOI]
- 7 Efficient genome editing in plants using a CRISPR/Cas system [DOI]
- 8 Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes [DOI]
- 9 Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA [DOI]
- 10 Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes [DOI]
- 11 DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins [DOI]
- 12 Programmable RNA recognition and cleavage by CRISPR/Cas9 [DOI]
- 13 Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts [DOI]
- 14 Directed evolution of CRISPR-Cas9 to increase its specificity [DOI]
- 15 Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions [DOI]
- 16 A highly specific SpCas9 variant is identified by in vivo screening in yeast [DOI]
- 17 High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System [DOI]
- 18 DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks [DOI]
- 19 In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation [DOI]
- 20 Turning Meiosis into Mitosis [DOI]
- 21 Microhomology-based choice of Cas9 nuclease target sites [DOI]
- 22 Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR /Cas9 gene editing [DOI]
- 23 Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System [DOI]
- 24 A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice [DOI]
- 25 Transgenerational CRISPR-Cas9 Activity Facilitates Multiplex Gene Editing in Allopolyploid Wheat [DOI]
- 26 Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9 [DOI]
- 27 Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress [DOI]
- 28 Genome Editing in Cotton with the CRISPR/Cas9 System [DOI]
- 29 Elimination of Toxic Microsatellite Repeat Expansion RNA by RNA-Targeting Cas9 [DOI]
- 30 CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation [DOI]
- 31 Site-directed mutagenesis in Petunia × hybrida protoplast system using direct delivery of purified recombinant Cas9 ribonucleoproteins [DOI]
- 32 Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants [DOI]
- 33 Turning rice meiosis into mitosis [DOI]
- 34 Manipulating mitochondrial DNA heteroplasmy by a mitochondrially targeted restriction endonuclease [DOI]
- 35 Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus [DOI]
- 36 Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention [DOI]
- 37 Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2 [DOI]
- 38 Genome-wide tracking of dCas9-methyltransferase footprints [DOI]
- 39 Rapid and reversible epigenome editing by endogenous chromatin regulators [DOI]
- 40 Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries [DOI]
- 41 Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases [DOI]
- 42 CRISPR ‐S: an active interference element for a rapid and inexpensive selection of genome‐edited, transgene‐free rice plants [DOI]
- 43 YAO is a nucleolar WD40-repeat protein critical for embryogenesis and gametogenesis in Arabidopsis [DOI]
- 44 CRISPR/Cas9 genome editing of rubber producing dandelion Taraxacum kok-saghyz using Agrobacterium rhizogenes without selection [DOI]
- 45 Temperature effect on CRISPR-Cas9 mediated genome editing [DOI]
- 46 Programming sites of meiotic crossovers using Spo11 fusion proteins [DOI]
- 47 High efficiency of targeted mutagenesis in arabidopsis via meiotic promoter-driven expression of Cas9 endonuclease [DOI]
- 48 Advanced editing of the nuclear and plastid genomes in plants [DOI]
- 49 Generation of stable nulliplex autopolyploid lines of Arabidopsis thaliana using CRISPR/Cas9 genome editing [DOI]
- 50 DNA Epigenome Editing Using Crispr-Cas Suntag-Directed DNMT3A [DOI]
- 51 De novo domestication of wild tomato using genome editing [DOI]
- 52 A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells [DOI]
- 53 Domestication of wild tomato is accelerated by genome editing [DOI]
- 54 Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors [DOI]
- 55 Rapid improvement of domestication traits in an orphan crop by genome editing [DOI]
- 56 A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds [DOI]
- 57 Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes [DOI]
- 58 One-step genome editing of elite crop germplasm during haploid induction [DOI]
- 59 CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing [DOI]
- 60 Analysis and minimization of cellular RNA editing by DNA adenine base editors [DOI]
- 61 Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis [DOI]
- 62 Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding [DOI]
- 63 An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice [DOI]
- 64 CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors [DOI]
- 65 High-efficiency generation of fertile transplastomic Arabidopsis plants [DOI]
- 66 A Highly Efficient Cell Division-Specific CRISPR/Cas9 System Generates Homozygous Mutants for Multiple Genes in Arabidopsis [DOI]
- 67 Abscisic Acid Mediated Abiotic Stress Tolerance in Plants [DOI]
- 68 ZFN, TALEN and CRISPR-Cas9 mediated homology directed gene insertion in Arabidopsis: A disconnect between somatic and germinal cells [DOI]
- 69 CRISPR-Cas Mediated Genome Editing: A Paradigm Shift towards Sustainable Agriculture and Biotechnology [DOI]
- 70 Plant Pan-genomes: A New Frontier in Understanding Genomic Diversity in Plants [DOI]
- 71 Chromosomal Engineering through CRISPR– Cas Technology: A Way Forward [DOI]
- 72 Tapping into the Unsung Potential of CRISPR/CAS Technology in Agriculture [DOI]
Cited by 6
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Muslim Qadir, Navjot Kaur, Faiz Ur Rahman · Frontiers in Plant Science · 2026
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