Reduction of Salmonella enterica & Staphylococcus aureus Biofilm Development on Glass Tube by Plant Extracts

Abstract

The objective of the present study is to evaluate anti-biofilm effect of the water soluble plant extracts such Coccinia grandis, Terminalia arjuna, Centella asiatica against Salmonella enterica and Staphylococcus aureus biofilm. Crude water soluble extracts of respective plants with different concentration was evaluated against biofilm adopting glass tube. Then washed with crystal violet dye and PBS buffer for observing the ring formation. Biofilm inhibition study revealed water soluble plant extracts inhibited biofilm formation. In our experiment we found that the plant extracts Coccinia grandis, Terminalia arjuna & Centella asiatica gave excellent result for the reduction of biofilm of Salmonella enteric & Staphylococcus aureus. Water soluble Coccinia grandis extract is very effective for biofilm reduction than alcoholic C. grandis extracts. We measured the antiplantonic effect of these extracts by including the extracts into the nutrient agar media that containing respective organism by creating the hole into the plate then observed the result after 24-hour incubation. We also measured minimum inhibition concentration of these extracts through spectrophotometer with the help of the nutrient broth media. Bacterial motality was tested in petri plates with semi-solid medium (LB+0.4% agar) containing plant extracts and culture were inoculated in the center of the plate. This research will be very beneficial for us to reduce the pathogenic S, aureus & S. enterica biofilm by natural source especially plant extracts.