Quantification of Impurity-G Content in Levetiracetam Injection (100 mg/ml) by Using  RP-HPLC Technique

Syed Mastan Ali; Ponnuri Bharath; V. Siva Ramakrishna; D. Ramachandran

doi:10.9734/jpri/2022/v34i28B36035

Research Article Open access CC BY 4.0

Quantification of Impurity-G Content in Levetiracetam Injection (100 mg/ml) by Using RP-HPLC Technique

Syed Mastan Ali, Ponnuri Bharath, V. Siva Ramakrishna, D. Ramachandran

Journal of Pharmaceutical Research International · pp. 53–61 · Published 2 Apr 2022

10.9734/jpri/2022/v34i28B36035

Abstract

Extremely responsive technique for the quantification of impurity-G content in Levetiracetam samples by RP-HPLC method has been presented in the here manuscript. Quantification of impurity-G content in Levetiracetam sample by HPLC with UV Detector. Impurity-G was concluded by RP-HPLC technique by waters X-Terra MS-C18 (250 x 4.6mm, 5µm) column as motionless segment. Column oven preserved 45°C and taster cooler temperature 5°C, injection quantity 50µL, flow rate was 1.0 mL/min, impurity-G was detected using UV detector at the wavelength of 200nm and run time was 30 minutes. The movable segment utilized pH 3.0 buffer and acetonitrile in the proportion of (850:150 v/v) isocratic mode. The technique legalization has been carried as per International Conference on Harmonization guidelines (ICH). Limit of quantitation (LOQ) was found 0.4122 µg/mL for impurity-G.

Impurity-G Levetiracetam RP-HPLC method validation limit of quantitation

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Syed Mastan Ali, Ponnuri Bharath, V. Siva Ramakrishna, D. Ramachandran (2022). Quantification of Impurity-G Content in Levetiracetam Injection (100 mg/ml) by Using RP-HPLC Technique. Journal of Pharmaceutical Research International , 53–61. https://doi.org/10.9734/jpri/2022/v34i28B36035

Affiliations

Syed Mastan Ali — Acharya Nagarjuna University, Nagarjuna University, Guntur, A. P, India.
Ponnuri Bharath — Acharya Nagarjuna University, Nagarjuna University, Guntur, A. P, India.
V. Siva Ramakrishna — Acharya Nagarjuna University, Nagarjuna University, Guntur, A. P, India.
D. Ramachandran — Acharya Nagarjuna University, Nagarjuna University, Guntur, A. P, India.

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