In-vitro DPPH Free Radical Scavenging Activity of the Plant Murraya koenigii Linn (Curry Leaf) in Rajshahi, Bangladesh

Abstract

Aim: The use of herbal medicine is becoming popular day by day due to toxicity and side effects of allopathic medicines and hence the present study was carried out to assess the in-vitro DPPH free radical scavenging potential of the methanolic extract and their different fractions and aqueous extracts of Murraya koenigii Linn leaves. Materials and Methods: Considering the medicinal importance of the plant Murraya koenigii Linn (Curry leaf), antioxidant activity (AC), total phenolic content (TPC), total flavonoid content (TFC), of different fractions of methanolic extract (DIA-ion resin adsorbed fraction, chloroform, Ethyl acetate and petroleum ether) of M. koenigii were investigated. Results: Among the fractions, DIA-ion resin adsorbed fraction showed the highest total antioxidant activity with absorbance 2.320±06 and petroleum ether fraction showed the lowest total antioxidant activity with absorbance 1.944 at 100 mg/ml concentration. The TPC were found to be ranges from 13.285 to 17.52 mg GAE/g while highest amount of TFC (16.65 mg CatE/g) was found in different extracts of leaves. DPPH free radical scavenging activity of different extracts of leaves was also measured and their activity was observed in the following order: DAF>PE>EE>CE, with IC50 values from 15.53 µg/ml to 24.62 µg/ml.