Skip to content
Research Article Open access CC BY 3.0

Use of Palm Oil Mill Effluent as Medium for Cultivation of Chlorella sorokiniana

Charles Ogugua Nwuche, Doris Chidimma Ekpo, Chijioke Nwoye Eze, Hideki Aoyagi, James Chukwuma Ogbonna

Biotechnology Journal International · pp. 305–316 · Published 19 Feb 2014

10.9734/BBJ/2014/6921

Abstract

Aims: Palm oil mill effluent (POME) erodes the principal biophysical characteristic of both soil and water when discharged untreated but could be exploited as medium for microalgae cultivation due to its vast mineral contents. Place and Duration of study: POME samples were collected from a local palm oil processing mill at Nsukka, Enugu State, Nigeria. A part of the study was done at the Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan while the rest at the University of Nigeria, Nsukka between March and September, 2012. Methodology: Chlorella sorokiniana C212 was grown in several Batches (A-D) of POME supplemented with urea (60 mg/L) before subjecting to different sterilization protocols. Cultivation was conducted in shaker flasks at 150 rpm, 1 vvm, 3000 lux and pH 7.0±0.2. Results: The filter sterilized Batch (B) promoted the highest (1070±30 mg/L) dry cell weight (DCW), lipid (156±12 mg/g-cell) and chlorophyll (1.59±0.11 mg/g-cell) contents while chemical oxygen demand (COD) decreased by 45±08%. The autoclaved medium (Batch A) gave the least DCW (310±20 mg/L), lipid production (40±05 mg/g-cell) and chlorophyll content (0.58±0.02 mg/g-cell) while COD reduced by 20±04%. The highest COD decrease (70±05%) was achieved in the unsterilized Batch (D). Batch B was most positively affected by dilution because at 75% concentration, DCW increased to 1360±30 mg/L, lipid contents to 174±10 mg/g-cell, chlorophyll to 1.87±0.14 mg/g-cell the while COD declined by 63±03%. Conclusions: POME has potential for use in microalgae cultivation with significant saving in treatment costs.

Palm oil mill effluent microalgae cultivation chlorella sorokiniana sterilization chlorophyll chemical oxygen demand dilution

Cited by 27

A conceptual review on microalgae biorefinery through thermochemical and biological pathways: Bio-circular approach on carbon capture and wastewater treatment

Jayaseelan Arun, Kannappan Panchamoorthy Gopinath, PanneerSelvam SundarRajan · Bioresource Technology Reports · 2020

The feasibility of immobilized Chlorella vulgaris cultivated in palm oil mill effluent for lipid and fatty acid methyl ester production

Noor Raihana Abu Sepian, Nur Hidayah Mat Yasin, Norazwina Zainol · Materials Today: Proceedings · 2022

Greening the oil industry: Microalgae biorefinery for sustainable oil-produced water treatment and resource recovery

Mohammad Sibtain Kadri, Nayana K., Reda Fassi Fihri · Journal of Water Process Engineering · 2024

Advances in POME treatment methods: potentials of phycoremediation, with a focus on South East Asia

K. A. Tan, W. O. Wan Maznah, N. Morad · International Journal of Environmental Science and Technology · 2021

Enhanced growth and nutrients removal efficiency of Characium sp. cultured in agricultural wastewater via acclimatized inoculum and effluent recycling

Azianabiha A Halip Khalid, Zahira Yaakob, Siti Rozaimah Sheikh Abdullah · Journal of Environmental Chemical Engineering · 2016

Evaluation of some biological activities of Euglena gracilis biomass produced by a fed-batch culture with some crop fertilizers

Chijioke Nwoye Eze, James Chukwuma Ogbonna, Okechukwu Obisike Ndu · African Journal of Biotechnology · 2017

Showing 20 of 27 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

0

Page views

0

PDF downloads

0

Outbound clicks

27

Citations

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

No views recorded yet.

Traffic sources

Referring site, by host.

No traffic recorded yet.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.