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Research Article Open access CC BY 4.0

Protein Expression in Synechococcus PCC 7002: A Quantitative Comparison of Promoters and Integration Sites

Isabell Pappert, Simon Greulich, Franziska M. Joseph, Beate Otto, Ralf Kaldenhoff

Journal of Advances in Biology & Biotechnology · pp. 28–39 · Published 13 Jun 2023

10.9734/jabb/2023/v26i4629

Abstract

Photosynthetic Cyanobacteria can be used as a chassis for different synthetic biology approaches. However, quantitative comparison of tools for engineering, such as those for heterologous gene expression, is often not available. Here, we report the comparative quantification of heterologous protein production in Synechococcussp.PCC 7002 regarding protein expression cassettes and locations of foreign gene integration using sf-GFP as a reporter. We used promoter cpc560 as reference because it was described as a "super strong" promoter. sf-GFP-expression constructs were integrated into neutral sites NS_1, NS_2, NS_3 and the extrachromosomal plasmid pAQ1. The latter induced a sf-GFP level of approximately 10-fold in comparison to a reference promotor expression. Protein-fusion with 6xHis increased sf-GFP as well as expression of sf-GFP fusion with ß subunit of phycocyanin.

Cyanobacteria genomic integration GFP pAQ1 protein production Synechococcus sp PCC 7002

References (9)

  1. 1 Engineering and characterization of a superfolder green fluorescent protein [DOI]
  2. 2 Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria [DOI]
  3. 3 Development and optimization of genetic toolboxes for a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 [DOI]
  4. 4 Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803 [DOI]
  5. 5 Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number [DOI]
  6. 6 Cyanobacterial Production of Biopharmaceutical and Biotherapeutic Proteins [DOI]
  7. 7 Engineering of Synechococcus sp. strain PCC 7002 for the photoautotrophic production of light-sensitive riboflavin (vitamin B2) [DOI]
  8. 8 THE PRODUCTION OF HYDROGEN PEROXIDE BY BLUE-GREEN ALGAE: A SURVEY1 [DOI]
  9. 9 The Effect of Promoter and RBS Combination on the Growth and Glycogen Productivity of Sodium-Dependent Bicarbonate Transporter (SbtA) Overexpressing Synechococcus sp. PCC 7002 Cells [DOI]

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