Prevalence and Antimicrobial Resistance of Campylobacter Species Isolated from Backyard Chickens in Grenada, West Indies
Ravindra Sharma, Keshaw Tiwari, Vanessa Mathew Belmar, Sunil Kumar, Sagar M. Goyal, Victor A. Amadi, Natalie Watson, Harry Hariharan
Microbiology Research Journal International · pp. 1–8 · Published 10 Nov 2015
10.9734/BMRJ/2016/21741Abstract
Aim: This study was carried out to assess the prevalence of Campylobacter spp. in free range chickens in Grenada, West Indies and to determine the antimicrobial susceptibility of isolates. Study Design: Cloacal swabs were collected from 315 free range chickens from randomly selected households from all six parishes of Grenada between June and July 2014. Cloacal swabs were cultured for Campylobacter in the Pathobiology Diagnostic Laboratory, School of Veterinary medicine, St. Georges University Grenada. Isolates were further tested through PCR for speciation. Methodology: Standard culture methods for isolation of Campylobacter spp were used. Isolates were characterized by conventional phenotypic tests and confirmed by PCR using species specific primers. The 16s rRNA gene for Campylobacter spp.; the map A gene for C. jejuni and the ceuE gene for C. coli were selected for PCR. Isolates were tested through E-test for their antimicrobial susceptibility using Ampicillin, Ciprofloxacin, Chloramphenicol, Erythromycin, Gentamicin, Metronidazole and Tetracycline. Results: A total of 158 isolates (50.2%) were obtained by culture. PCR on 93 isolates identified 33 isolates as C. jejuni, 6 as C. coli and 54 as mixed infection with C. jejuni and C. coli. None of the isolates was resistant to chloramphenicol and erythromycin; susceptibility to other antimicrobials varied among isolates. Multidrug resistance was high in C. coli (33.3%), followed by mixed infection isolates (22.2%) and C. jejuni (12.0%). Conclusion: Results of the study show that approximately 50% of backyard chickens in Grenada harbor Campylobacter spp. These backyard chickens pose a great risk for humans as hazard analysis critical control point (HACCP) is not observed during the slaughter and processing of these chickens.
References (20)
- 1 Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations
- 2 Epidemiologic and Clinical Features ofCampylobacter jejuniInfections [DOI]
- 3 Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli [DOI]
- 4 Contamination of chicken carcasses in Gauteng, South Africa, by Salmonella, Listeria monocytogenes and Campylobacter [DOI]
- 5 Comparison of Broth Microdilution, E Test, and Agar Dilution Methods for Antibiotic Susceptibility Testing of Campylobacter jejuni and Campylobacter coli [DOI]
- 6 CampylobacterandArcobacter [DOI]
- 7 Comparison of Antimicrobial Susceptibility Testing of Campylobacter spp. by the Agar Dilution and the Agar Disk Diffusion Methods [DOI]
- 8 Pet Dogs and Chicken Meat as Reservoirs ofCampylobacterspp. in Barbados [DOI]
- 9 Skim milk enhances the preservation of thawed − 80 °C bacterial stocks [DOI]
- 10 Campylobacter in Food Animals and Humans in Northern Thailand [DOI]
- 11 Prevalence and Comparison of Genetic Profiles of Campylobacter Strains Isolated from Poultry and Sporadic Cases of Campylobacteriosis in Humans [DOI]
- 12 Pre-harvest surveillance of Campylobacter and Salmonella in Danish broiler flocks: a 2-year study [DOI]
- 13 Antimicrobial susceptibilities of thermophilic Campylobacter from humans, swine, and chicken broilers.
- 14 Resistance patterns of Campylobacter spp. strains isolated from poultry carcasses in a big Swiss poultry slaughterhouse [DOI]
- 15 Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone inCampylobacterIsolates from Sheep and Goats in Grenada [DOI]
- 16 The role of management systems in the epidemiology of thermophilic campylobacters among poultry in Eastern zone of Tanzania [DOI]
- 17 Molecular characterization of Campylobacter isolated from chickens and humans in northern Thailand.
- 18 Fluoroquinolone and metronidazole resistance of Campylobacter spp from broiler chickens and antimicrobial use on farms in Grenada, West Indies
- 19 Campylobacter coli and C. jejuni isolated from farmed pigs in Grenada, West Indies and their antimicrobial resistance patterns
- 20 Comparative analysis of cultural isolation and PCR based assay for detection ofCampylobacter jejuniandCampylobacter coliin foods of animal origin [DOI]
Cited by 8
Bhumika K. Sharma, Katelyn Thille, Vanessa Matthew Belmar · PLoS ONE · 2020
Victor A. Amadi, H. Hariharan, K. Tiwari · 2018
L. Coad, John E. Fa, K. Abernethy · 2017
A. Karikari, K. Obiri-Danso, E. Frimpong · BioMed Research International · 2017
R. Vivekanandhan, Z. Dubal, P. Muralikrishna · 2020
S. Shamim, S. Gul, A. Khan · Pharmaceutical Chemistry Journal · 2022
Hazeeq Azman, Saleha Abd Aziz, Abdul Latif Ibrahim · AIP Conference Proceedings · 2023
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