Preparation of Mouthwash using Banana Stem Extract Mediated Copper Nanoparticles and Its Cytotoxic Effect – An In-vitro Study

Abstract

Background: Copper nanoparticles have recently attracted researchers due to their low cost, availability and other novel properties. Various parts of Musa sapientum which belong to the family Musaceae have been used for various medicinal purposes. The aim of the study is to assess the cytotoxic effect of mouth wash prepared using banana stem mediated copper nanoparticles. Materials and Methods: 100 mL of distilled water is mixed with 1g of banana stem extract and distilled. The filtrate is mixed with 0.477g of copper sulphate mixed in 60 mL of distilled water. There were a total of 10 shrimps per dilution. The artificial seawater upto 10 mL per test tube is controlled. The test tube was left uncovered under a lamp. The number of surviving shrimps were counted and recorded after 24 h. The percentage of motility was calculated by dividing the total number of dead nauplii by the total number and then multiplied by 100%. This is to ensure that the death of nauplii is attributed to the compounds present in nanoparticles. Results: Percentage of lethality for the banana stem extract mediated copper nanoparticles mouthwash at concentrations of 5 μL, 10 μL, 20 μL, 40 μL, 80 μL were 60%, 70%, 100%, 100%, 100% respectively. Conclusion: Thus, with an increase in concentration of the banana stem mediated copper nanoparticles mouthwash, the cytotoxic activity increases. Thus from the study, it is evident that natural mouthwash can be prepared with banana stem mediated copper nanoparticles with less side effects.