Preparation of Methanolic Crude Extract of Padina Gymnospora Seaweed and their Anticancer Activities against Cancer Cell Line

Abstract

Background: Cancer is a major health problem worldwide and still lacks fully effective treatments. As a result, natural-products-based alternative medicines have been developed. Marine algae are a vital part of the marine environment, with high biodiversity and contain a diverse range of useful chemicals. Aim: Aim of the study was to assess the anticancer activity of Padina gymnospora crude extract against lung cancer cell line Materials and Methods: The Seaweed P. gymnospora was evaluated for its anti-cancer activity via MTT assay, further, morphological study of the cells was done to check its efficacy. Finally, the results were analyzed by Student's-t-test using MS-Excel, represented as mean ± SD for triplicates. The results were computed statistically (SPSS/10 Software Package; SPSS Inc., Chicago) using one-way ANOVA. The level of statistical significance was set at p<0.05. Results and Discussion: The results of this study indicate that P. gymnospora has significant anticancer activity. At the highest concentration, 500μl, methanolic crude extracts of P. gymnospora showed the maximum anticancer activity, where the cell viability was only 16.41+7.15. The morphological study also revealed that maximum cell death had occurred in the maximum concentration of the methanolic crude extract of P. gymnospora. Conclusion: Despite the widespread use of algae-derived compounds and extracts in the food industry, there are still limited anticancer drugs available in the industry. Thus, it is imperative that new drug discovery programs using seaweeds with a much more mechanistic approach are needed.