Preparation and Cytotoxic Effect of Pterocarpus santalinus Selenium Based Mouthwash

Abstract

Introduction: A mouthwash or rinse cannot replace the regular oral hygiene routine of twice-daily tooth brushing and daily flossing.A mouthwash or rinse freshen breath, and control the bad breath.A mouthwash is a medicated liquid which is held in the mouth and swished by the action of perioral musculature to eliminate the oral pathogens.The medicinal plants have their application in pharmaceutical, cosmetic, agricultural and food industries.Medicinal plants act as a natural source for bioactive medicinal compounds.Selenium is a vita micronutrient that has excellent antimicrobial, anti cancerous, antidiabetic, and anti-inflammatory properties.The biological method of synthesis of Selenium nanoparticles has extensive application in the field of biomedicine due to low toxicity, targeted delivery of Nano drugs and stability. Materials and Methods: Pterocarpus santalinus plant extract and mouthwash was prepared and cytotoxicity activity was performed. Results: On day 1 all the 10 nauplii were alive in all the concentrations. On day 2 when the concentration was 5, 10, 20, 40,80, control the number of live nauplii was 10,8,8,8,7,10 respectively.Results were tabulated and graphically analyzed in SPSS software. Correlation analysis was done using SPSS software. Conclusion: Increased concentration of selenium nanoparticles was not toxic to living nauplii as it did not cause the death of it.But the Pterocarpus santalinus extraction based selenium nanoparticles have less to no cytotoxicity.This study shows that mouth rinses from medicinal plants as a source of an excellent alternative with less common side-effects to chemical-based oral antimicrobial products.