Skip to content
Research Article Open access CC BY 4.0

Prediction of Solanum lycopersicum Target of Rapamycin (SlTOR) Protein

Ding Li-Na, Wang Rui, Zhang Jun, Wang Hao-Ran, Wang Xiao-Yan, Yu Lan, Cui Na

Biotechnology Journal International · pp. 1–11 · Published 31 Mar 2020

10.9734/bji/2020/v24i230097

Abstract

Aims: Tomato (Solanum lycopersicum) is an important protected vegetable in China. Its yield and quality receive much concern, however, its growth is often adversely affected by environmental stress, and so improving stress-resistance of tomato has become an urgent issue to be resolved in facility cultivation. Recent studies find that the TOR(Target of Rapamycin) complex acts as a central coordinator of energy, growth, hormones and stress signals, as well as plays a critical role in regulating transcription, protein synthesis, cell size, cell division, and basal metabolism. To study the mechanism of SlTOR in tomato growth and development as well as in stress responses, we did a series of bioinformatics analysis on SlTOR. Study Design: In order to explore the mechanism of TOR in regulating tomato resistant to adverse conditions, we systematically analyzed the SlTOR gene with bioinformatics methods, and carried out the determination of its tissue-differential expression, aiming at laying down the basis for further experiment research. Place and Duration of Study: College of Bioscience and Biotechnology, between March 2018 and April 2019. Methodology: Bioinformatics analysis was conducted by online programmes. The expression of SlTOR gene in different tissues of tomato was determined by qRT-PCR. Results: Our results showed that SlTOR was an evolutionarily conserved protein kinase, of which the molecular formula was C12366H19734N3490O3584S104, the relative molecular weight was 277978.19Da and the number of amino acid residues was 2470. Besides, it was predicted to be an acidic and unstable protein. SlTOR protein did not contain the signal peptide or transmembrane region, showing that it might be an intracellular protein. And SlTOR was speculated to be targeted to the chloroplast. Moreover, SlTOR had five domains including HEAT, FAT, PIKKc_TOR, FRB and FATC. The KEGG database displayed the only one SlTOR metabolism pathway related to autophagy. The STRING database found that SlTOR probably interacted with SlSnRK1 and SlPP2C. The experimental results of the expression of SlTOR gene in different tissues suggested that in the mature tomato plant, it was expressed the most highly in the root, followed by in the fruit let and in the mature fruit. Our experimental results were roughly consistent with the predicted results.

Tomato TOR ABA SnRK protein kinase stress response

References (22)

  1. 1 A revised set of potentials for β‐turn formation in proteins [DOI]
  2. 2 Reciprocal Regulation of the TOR Kinase and ABA Receptor Balances Plant Growth and Stress Response [DOI]
  3. 3 Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid [DOI]
  4. 4 Quantitative phosphoproteomics identifies SnRK2 protein kinase substrates and reveals the effectors of abscisic acid action [DOI]
  5. 5 Expression and disruption of the Arabidopsis TOR (target of rapamycin) gene [DOI]
  6. 6 Bioactivities of phytochemicals present in tomato [DOI]
  7. 7 Arabidopsis TARGET OF RAPAMYCIN Interacts with RAPTOR, Which Regulates the Activity of S6 Kinase in Response to Osmotic Stress Signals [DOI]
  8. 8 ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis [DOI]
  9. 9 Rapamycin and Glucose-Target of Rapamycin (TOR) Protein Signaling in Plants [DOI]
  10. 10 TOR Is a Negative Regulator of Autophagy in Arabidopsis thaliana [DOI]
  11. 11 The Role of Target of Rapamycin Signaling Networks in Plant Growth and Metabolism [DOI]
  12. 12 mTOR: a protein kinase switching between life and death [DOI]
  13. 13 SnRK1 activates autophagy via the TOR signaling pathway in Arabidopsis thaliana [DOI]
  14. 14 Trehalose-6-phosphate and SnRK1 kinases in plant development and signaling: the emerging picture [DOI]
  15. 15 Translatome analysis of an NB-LRR immune response identifies important contributors to plant immunity in Arabidopsis [DOI]
  16. 16 Target of Rapamycin kinase: central regulatory hub for plant growth and metabolism [DOI]
  17. 17 Cloning, bioinformatics and transcriptional analysis of caffeoyl-coenzyme A 3-O-methyltransferase in switchgrass under abiotic stress [DOI]
  18. 18 14-3-3 proteins regulate Tctp–Rheb interaction for organ growth in Drosophila [DOI]
  19. 19 [Regulation of different calcium forms on the photosynthesis of tomato leaves under heat stress].
  20. 20 Linking nutrients to growth [DOI]
  21. 21 Bioinformatic Analysis on Jellyfish Hematoxin [DOI]
  22. 22 Genetic improvement in tomato ( Solanum lycopersicum ) against salt stress

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

64

Page views

0

PDF downloads

28

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.