Preclinical Evaluation of Antidotal Property of Mritasanjeevana agada in Poisoning- A Study Protocol

Abstract

Background: As the poisoning is becoming a threat to rural India, it is necessary to increase the survival time to avail the primary treatment. For the treatment of poisoning, Agada is described in Ayurveda as an antidote. Agada is a polyherbal or herbomineral formulation constituted with combination antitoxic drugs along with some antioxidant, immunomodulator or hepatoprotective drugs. But they need to be revalidated for their efficacy and safety on the basis of contemporary assessment parameters Aim: Evaluation of antidotal property of Mritasanjivana Agada in poisoning. Objectives: • To increase the survival time after the administration of Mritasanjivana Agada in snake venom and aluminium phosphide poisoning in albino mice. • To compare the efficacy of Mritasanjivana Agada and Anti-snake venom as an antidote. • To standardize the Mritasanjivana Agada. Methodology: Mritasanjeevana Agada will be prepared and standardized. Cobra venom poisoning and aluminium phosphide poisoning have been selected as the representative for the animate poison and artificial/ synthetic poison. After inducing poisoning in mice, one group will receive its standard antidote and other will receive standard antidote with Mritasanjivana Agada. The third group will receive only Mritasanjivana Agada without its standard antidote. All the groups will be assessed on the basis of hematology, biochemistry, Superoxide dismutase (SOD) level, Malondialdehyde (MDA) level and histopathology in case of death of the animals. Results: Mritasanjeevana agada is expected to increase the survival time in the treatment of snake venom and aluminium phosphide poisoning in albino mice. Conclusion: Mritasanjeevana agada may be as efficacious as Anti-snake venom as an antidote.