Evaluation of Ameliorative Potentials of Cleanshield Liquid Supplement in Testosterone Induced Benign Prostatic Hyperplastic (BPH) Rat Model

Abstract

Aim: The aim of this study was to evaluate the ameliorative effect of Cleanshield Liquid Supplement in Testosterone Induced Benign Prostatic Hyperplasic (BPH) in male albino rats Study Design:  This study is a case controlled experimental study. Place and Duration of Study: Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria, between December, 2019 and May, 2020. Methodology: A total of 30 male albino wistar rats were used for this study and divided into 6 groups of 5 rats each. Testosterone propionate (4 mg/kg) was used to induced BPH subcutaneously in the rats and then were given (0.5 mg/kg) dutasteride, an anti-BPH drug and Cleanshield liquid supplement (0.24ml, 0.48ml and 0.72ml) for 30 days. At the end of the 30 days treatment, the animals were sacrificed. Chloroform was used to anaesthetize the rats and 5 ml of whole blood samples were collected through cardiac puncture at the end of 8 hours fast. The blood samples collected were separated and the serum was used to analyze for prostate specific antigen (PSA) using rat specific PSA ELISA kit produced by Shanghai Korain Biotech Co., Ltd, China, while liver enzymes (alanine aminotransaminase (ALT), aspartate aminotransaminase (AST) and alkaline phosphatase (ALP)) were analyzed using spectrophotometric method. Liver tissues of the rats were excised and used for histological analysis. SPSS version 22.0 was used for statistical analysis and p<0.05 was considered statistically significant. Results: The results showed that comparison between the Cleanshield treated groups and the Avodart group (group 3) showed no significant difference but there was a non-significant reduction in the mean PSA values of all the clean shield treated group with the group that received the highest amount of Cleanshield having the most reduced mean PSA value. The mean PSA value of the negative control group (NC) compared to dutasteride group and all Cleanshield group showed statistically significant difference. The mean ALT values did not show any significant difference statistically when the groups that received Cleanshield supplement were compared to PC group. In the mean values of AST, only the comparison between anti BPH group and the group that received 0.72ml of Cleanshield produce statistically significant difference (p=0.036). That of the ALP comparison between the mean ALP values of the PC group (grp 2) showed significant difference against group 2(p=0.001) and against group 5 (p=0.003). Avodart group (group 3) showed a statistical difference when compared to group 4(0.24 ml of Cleanshield) at p=0.000. Group 4(0.24 ml of Cleanshield) showed statistical difference when compared to group 5(0.48ml of Cleanshield) at p=0.001. Conclusion: Conclusively, Cleanshield liquid supplement is not toxic to the liver but does not possess significant BPH ameliorative potentials.