Production and Optimization of Cellulase from Penicillium sp. Using Corn-cob and Pawpaw Fibre as Substrates
Francis Sopuruchukwu Ire, Augustine Onwuchekwa Okoli, Victor Ezebuiro
Journal of Advances in Microbiology · pp. 1–10 · Published 5 Feb 2018
10.9734/JAMB/2018/39227Abstract
Production and Optimization of Cellulase from Penicillium sp. Using Corn-cob and Pawpaw Fibre as Substrates Aim: This study reports the production and optimization of cellulase from Penicillium sp. using corn-cob (CC) and pawpaw fibre (PF) as substrates. Methods: Nine fungal isolates, obtained from compost soil, were screened for cellulolytic activity. Isolate CPF-1, based on its ability to give the highest zones of clearance and cellulolytic activity, was selected. CPF-1 was identified as Penicillium sp. based on its cultural and morphological characteristics. Cellulase activity was determined by the DNS method on Congo red agar plate. Effects of temperature, pH and metal ions (Zn2+, Hg2+, Fe2+, Mg2+, Ca2+ and Co2+) on crude cellulase activity and stability were studied using two substrates (corn-cob and pawpaw fibre) by solid state fermentation. Results: Data obtained from the study revealed that the optimal pH and temperature values for the production of crude cellulase by the Penicillium sp. were pH 5 and 30°C, respectively; with maximum cellulase activity of 37.32 IU/mL. Optimum cellulase productivity of 15.787 IU/mL was obtained with CC as the substrate while 2.141 IU/mL was obtained with PF substrate after 1 h of fermentation. The cellulase produced was most stable at pH 5 and temperature of 40°C. Fe2+ and Co2+ were able to stimulate cellulase activity whereas the other ions inhibited the enzyme activity. Conclusion: This study has revealed the potentials of corn-cob and pawpaw fibre as substrates for cellulase production by Penicillium sp. through solid state fermentation (SSF); with corn-cob as the most suitable substrate. Considering that these substrates are readily available, they present cheaper substrate alternatives for potential large-scale cellulase production.
Cited by 12
Nattha Lojananan, B. Cheirsilp, Rawitsara Intasit · Bioresource Technology · 2024
Marta Maria Oliveira dos Santos Gomes, Izadora Santos Nicodemos, Monizy da Costa Silva · Preparative Biochemistry & Biotechnology · 2023
N. Srivastava, Rajeev Singh, Akbar Mohammad · Journal of food microbiology · 2022
J. A. Elegbede, V. A. Ajayi, A. Lateef · Environmental Technology & Innovation · 2021
P. C. Oliveira, A. R. de Brito, A. B. Pimentel · Revista Mexicana de Ingeniería Química · 2019
M. Subhosh Chandra, P. Suresh Yadav, P. V. Bramhachari · 2021
Marta Maria Gomes, Izadora Santos Nicodemos, Monizy Costa Silva · 2023
Ayoola E. Afe, Olusola T. Lawal, Olufemi S. Bamidele · Microbial Cell Factories · 2025
Serafín Perez Contreras, Ricardo Hernandez Martínez, Francisco Hernandez Rosas · Tropical and Subtropical Agroecosystems · 2023
Ayoola Ebenezer Afe, Olusola Tosin Lawal, Bukola Rukayat Oyelere · 2023
Related research
- Broad-range pH/Temperature-stable Cellulase from a Novel Hydrocarbon Contaminated Mangrove soil Bacterium, Bacillus licheniformis VVA21 — shares topic coverage
- Effect of Biochar on the Abundance of Soil Bacteria — shares topic coverage
- Bioprospecting of Cellulase Producing Extremophilic Bacterial Isolates from India — shares topic coverage
- Purification and Characteristics of Beta-1, 4-Glucanase Occel9 of Oxya chinensis — shares topic coverage
- Measurement of Cellulolytic Potential of Cellulase Producing Bacteria — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
12
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.