Phytochemistry and Antimicrobial Activities of Crude and Diluted Leaves and Roots Extracts of Uvaria chamae on Selected Bacteria
Ogochukwu Mary-Theodora Barbara Ochiabut, Bright Chukwuebuka Unaeze, Edna Ifeoma Chukwura, Ngozichukwu Gertrude Uzoewulu
Journal of Pharmaceutical Research International · pp. 7–18 · Published 6 Feb 2022
10.9734/jpri/2022/v34i8A35473Abstract
Plant metabolites are antibacterial. Antibacterial properties of aqueous, methanolic and n-hexane extracts of crude and diluted leaves and roots extracts of Uvaria chamae were studied to demonstrate its potential as achemotherapeutic agent. Test organisms include: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella typhi and Proteus vulgaris. Fresh tender leaves and roots of Uvaria charmae were collected, air- dried, grounded and soaked in methanol, n-hexane and aqueous solvents. Phytochemistry was done using standard procedures, antimicrobial activity was determined using agar-well diffusion method (punch hole) and MIC determined. Qualitative phytochemical analysis confirmed the presence of alkaloids, tannins, terpernoids, glycosides, saponins and flavonoids. Antimicrobial analysis of the plant parts using disc-diffusion method showed ten (10mg/ml) MIC concentration for aqueous extract of the leaves showed no activity, while root decoction inhibited only Salmonella typhi (14.00+0.47mm). Methanolic crude extracts of the leaves (UMLc) (400µg) exhibited antibacterial effects on all tested organisms, higher for Pseudomonas aeruginosa (23.67+ 0.27mm) than gentamycin(10µg) control (21.33+ 0.72) mm, while its 10-1 (40mg) dilution was active against Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa. Only crude n-hexane leaf extract (UNLc) was active for Pseudomonas aeruginosa (13.00 + 0.47mm). Crude methanolic extract of the roots UMRc(400µg) was active against all tested bacteria and clearance for Proteus vulgaris (23.33±0.54mm) was greater than that in both controls (ciprofloxacin (5 µg) (22.33±0.72 mm) and (gentamycin 10µg) (16.67±0.72mm).UNRchad activity against S. aureus, B. subtilis and P. aeruginosa(25.00 0.47 mm, 22.00 mm, and 12.67 0.27mm), while its 10-¹ dilution (40mg) had effects on Bacillus subtilis and Staphylococcus aureus (11.00 0.82mm, 13 0.47mm) only. The 10-2 (4µg) dilutions of (UMR and UNR) had no activity. Comparison of inhibition size between UML (20.33+ 0.27mm) and UMR (23.33+ 0.54mm)against Proteus vulgaris was significantly greater than inhibition size of gentamycin(10µg) (16.67+ 0.72) p<0.05.Activity of UML against Pseudomonas aeruginosa(23.67+ 0.27mm) was comparable to both ciprofloxacin (25.33+ 1.15mm) and gentamycin (21.33+ 0.72mm) p>0.05.Aqueous extracts of Uvaria chamae roots could be modified and harnessed for eradicating Salmonella typhi, UMLc and UMR for Proteus vulgaris and UML for Pseudomonas aeruginosa. Uvaria chamae crude and diluted extracts of both parts are potential future antibiotics.
References (16)
- 1 Plant Products as Antimicrobial Agents [DOI]
- 2 Antibiotics in Laboratory Medicine. [DOI]
- 3 Medicinal Plants and Traditional Medicine in Africa [DOI]
- 4 Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes [DOI]
- 5 Avoiding pitfalls in determining antimicrobial activity of plant extracts and publishing the results [DOI]
- 6 Phytochemical Composition and Biological Activities of Uvaria chamae and Clerodendoron splendens [DOI]
- 7 Annual Plant Reviews, Biochemistry of Plant Secondary Metabolism
- 8 Some determinant factors of antimicrobial susceptibility pattern of plant extracts [DOI]
- 9 A review: secondary metabolites of Uvaria chamae p. Beauv. (Annonaceae) and their biological activities [DOI]
- 10 Comparative assessment of antibacterial activity of uvaria chamae parts [DOI]
- 11 Modulation of inflammatory pathways, medicinal uses and toxicities of Uvaria species: potential role in the prevention and treatment of inflammation [DOI]
- 12 Evaluation of in-vivo anti-Salmonella activity of Uvaria chamae, Lantana camara and Phyllantus amarus used in Benin, West Africa [DOI]
- 13 Safety assessment of Uvaria chamae root extract: acute and subchronic toxicity studies
- 14 Effect of Oral Administration of the Leaf Extract of Uvaria chamae (Mmimi Ohia) in Albino Wistar Rats [DOI]
- 15 Comparative study on the levels of phytochemicals present in n-hexane, ethanol, aqueous, ethylacetate and methanol extracts of Uvaria chamae stem
- 16 Organic Chemistry: Current Research [DOI]
Cited by 3
Eskyl Togbé, Hornel Koudokpon, Aimé Césaire Ayéna · 2024
Showing 1 of 3 known citations — external sources report more than can currently be individually listed.
Related research
- Review: Parkia speciosa as Valuable, Miracle of Nature — shares topic coverage
- Urinary Pathogens amongst Parturients at the University of Port Harcourt Teaching Hospital, South-South, Nigeria — shares topic coverage
- Antimicrobial Studies on Leaf and Stem Extracts of Solanum erianthum — shares topic coverage
- An in-vitro Analysis of the Antioxidant and Antimicrobial Properties of the Methanol Extract of Hypoxis hemerocallideacorm (MEHHC) from Botswana — shares topic coverage
- Evaluation of Antimicrobial Potency and Phytochemical Screening of Persea americana Leaf Extracts against Selected Bacterial and Fungal Isolates of Clinical Importance — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
3
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.