Phytochemicals, Nutritional Analysis and In vitro Antioxidant Activities of Pickled Perilla frutescens Ethanolic Leaf Extract
Yongfu Li, Jiangning Gong, Chan Liu, Zhengwu Wang, Yan Wu, Yanling Gao, Jinhong Wu
European Journal of Medicinal Plants · pp. 303–314 · Published 27 Dec 2013
10.9734/EJMP/2014/7453Abstract
Aims: With pickled perilla leaves as raw materials, this paper proposed the optimal ethanol extraction conditions and made a profound analysis for extract in the compositions of major active ingredients, nutrients, mineral elements and amino acid to characterize the nutritional and biological properties of pickled perilla leaves, which could aid its finely processing and future application in the development of functional food. Methods: The optimum ethanol extraction process for preparing freeze-dried powder from pickled perilla was studied by means of orthogonal experiments, with the concentration of ethanol, extracting temperature and extracting time as factors. Meanwhile, the contents of the activity components, such as polysaccharide, flavones and rosmarinic acid, as well as the mineral elements and nutritional contents in the freeze-dried powder were determined according to the methods reported by relevant literatures without or with a few modifications. Results: The optimal extracting conditions as follows: 50ºC of temperature, 60 min of extraction time and 80% of ethanol concentration. Under the optimal extracting conditions, the extraction rate of the freeze-dried powder was 1.71%. Moreover, perilla leaf extract contained rich biological and nutritional ingredients, including 33.39% of flavonoids, 9.24% of polysaccharides, 22.79% of rosmarinic acid, 5.47% of protein, 7.61% of fat, 2354 mg/kg of Ca, 111.4 mg/kg of Fe, 5.045 mg/kg of Zn, 1817 mg/kg of K , 12.66 mg/kg of Mn and nine of essential amino acids. In addition, perilla leaf extract exhibited obvious scavenging effects on the DPPH•, •OH and O2-•. Conclusion: In summary, pickled perilla leaf ethanol extract was rich in biological ingredients as well as a variety of nutrients, and showed antioxidant activities in vitro, thus it is valuable and promising in the development of functional foods in the future.
References (15)
- 1 Antioxidant Activity and Mechanism of Protocatechuic Acid in vitro [DOI]
- 2 ORAC and DPPH assay comparison to assess antioxidant capacity of tea infusions: Relationship between total polyphenol and individual catechin content [DOI]
- 3 Rosmarinic acid, a major polyphenolic component of Perilla frutescens, reduces lipopolysaccharide (LPS)-induced liver injury in d-galactosamine (d-GalN)-sensitized mice [DOI]
- 4 IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF RAW PEPINO FRUIT (SOLANUM MURICATUM AITON)
- 5 Extract of Perilla frutescens Enriched for Rosmarinic Acid, a Polyphenolic Phytochemical, Inhibits Seasonal Allergic Rhinoconjunctivitis in Humans [DOI]
- 6 Hypolipidemic and antioxidant effects of total flavonoids of Perilla Frutescens leaves in hyperlipidemia rats induced by high-fat diet [DOI]
- 7 Growth inhibitory and apoptosis inducing effect of Perilla frutescens extract on human hepatoma HepG2 cells [DOI]
- 8 Biochemical Composition and Changes of Extracellular Polysaccharides (ECPS) Produced during Microphytobenthic Biofilm Development (Marennes-Oléron, France) [DOI]
- 9 Spectrophotometric determination of rosmarinic acid in plant cell cultures by complexation with Fe2+ ions [DOI]
- 10 Determination of total flavonoids in three Sedum crude drugs by UV-Vis spectrophotometry [DOI]
- 11 Effect of Perilla frutescens on nitric oxide production and DNA synthesis in cultured murine vascular smooth muscle cells [DOI]
- 12 Laboratory-scale extraction and characterization of ice-binding sericin peptides [DOI]
- 13 Evaluation of the antipyretic activity of Gypsum Fibrosum and its constituents
- 14 Antitumor Promoters from Edible Plants [DOI]
- 15 Antioxidant activity detection of radix Ligusticum, radix Rhodiolae, and radix Astragali by using DPPH method
Cited by 10
Miaw-Ling Chen, Chi-Heng Wu, Li-Shiuan Hung · Evidence-Based Complementary and Alternative Medicine · 2015
Nagaraj Basavegowda, Kanchan Mishra, Yong Rok Lee · RSC Adv. · 2014
Akriti Dhyani, Rajni Chopra, Meenakshi Garg · Biomedical and Pharmacology Journal · 2019
ChunFang Song, Xiaotong Ma, Jiaoling Wang · Journal of Food Processing and Preservation · 2021
TRA DONG PHUONG, LE THI MONG VUONG, QUACH NGO DIEM PHUONG · Science and Technology Development Journal - Natural Sciences · 2020
Showing 5 of 10 known citations — external sources report more than can currently be individually listed.
Related research
- In vitro Screening of Antibacterial and Antioxidant Activities of Essential Oils from Four Moroccan Medicinal Plants — shares topic coverage
- In vitro Antioxidant Activities and Effect of Hydroethanolic and Aqueous Extracts of Terminalia avicennioides (Combretaceae) on Salmonella — shares topic coverage
- Screening, Isolation and Identification of High Antioxidant Strains from 16 Kinds of Mushrooms — shares topic coverage
- Antioxidant and Antimicrobial Activities of Various Extracts from Safou (Dacryodes edulis) Cultivated in Côte d’Ivoire — shares topic coverage
- Screening of Antioxidant, Antibacterial and Antileishmanial Activities of Salvia officinalis L. Extracts from Morocco — shares topic coverage
Article metrics
Real usage data collected on this platform.
17
Page views
0
PDF downloads
1
Outbound clicks
10
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.