Phytochemical Investigation and Thin Layer Chromatography of Methanol Extract of Psoralea corylifolia and Emblica officinalis Leaves

Abstract

The current study focuses on phytochemical screening of Psoralea corylifolia and Emblica officinalis leaf extracts in methanol solvent. The antibacterial compounds found in both leaf extracts of Psoralea corylifolia and Emblica officinalis plants were investigated using phytochemical studies. The extracts contained flavonoids, terpenoids, tannins, alkaloids, saponins, and phenolic chemicals, according to preliminary phytochemical screening. The solvent systems of toluene: ethyl acetate: methanol (24:5:2) and chloroform: ethyl acetate: acetic acid (50:50:1) yielded the most phytochemicals from methanolic extracts of Psoralea corylifolia and Emblica officinalis plants, respectively. On TLC plates, these chemicals were separated, resulting in the discovery of different spots in both leaf extracts. The Rf values of methanol extract of P. corylifolia run under toluene: ethyl acetate: methanol (24:5:2) solvent system was 0.12, 0.19, 0.30, 0.41, 0.53, 0.65, 0.77, 0.84, 0.89, and 0.92, respectively, while Rf values of methanol leaf extract of E. officinalis run under chloroform: ethyl acetate: acetic acid (50:50:1)solvent system was 0.23, 0.31, 0.41, 0.64, 0.76 and 0.88 respectively. The results of the investigation will be used to confirm the proper identification of antibacterial fractions from P. corylifolia and E. officinalis crude plant extracts by GC-MS. The optimum solvent for extracting antibacterial components from P. corylifolia and E. officinalis leaves was methanol.