Phytochemical Investigation and Determination of Total Phenols, Flavonoid and Alkaloid Concentration in Leaves Extract of Miliusa tomentosa

Abstract

The genus Miliusa (Annonaceae) has over 60 species that are native to India, Bhutan, Australia, and New Guinea, but are mostly found in numerous Asian nations such as Vietnam, Thailand, and China. The growing interest in secondary metabolites' potent biological action highlighted the need of assessing their presence in therapeutic plants. Miliusa tomentosa (Roxb.) J. Sinclair (Annonaceae, M. tomentosa) is also known as hoom, kari. It is a huge deciduous tree that may reach a height of 20 metres. The bark is a dark brown. The leaves are thick leathery, ovate, oblong, 4-10 cm long, 2-5.5 cm broad, smooth above, gently hairy below, base rounded, border whole, tip pointy, and the leaf-stalk is 2-5 mm long. They are burned, and the smoke is allowed to travel over the baby's body after birth to minimise swelling. In the summer, children are fed fruits to help them recover from their frailty. The current study's goal is to look at the phytochemical profile of M. tomentosa leaf. The well-known test procedure available in the literature was used to determine the qualitative analysis of various phytochemical elements as well as the quantitative analysis of total phenolics, flavonoids, and alkaloids. The phenolic and flavonoids were quantified using the Folins Ciocalteau reagent technique and the aluminium chloride method, respectively. The presence of alkaloids, glycosides, flavonoids, phenols, proteins, carbohydrates, and saponins was discovered by phytochemical study. The present study concluded that the crude extract of M. tomentosa is a rich source of secondary phytoconstituents which impart significant antioxidant potential. The findings of the present study will be helpful to phytochemists, pharmacologists and pharmaceutical industries.