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Research Article Open access CC BY 3.0

Phytochemical Compositions and In vitro Antioxidant Capacity of Methanolic Leaf Extract of Axonopus Compressus (P. Beauv.)

Bartholomew O. Ibeh, Ezeja Maxwell, Habu Josiah Bitrus

European Journal of Medicinal Plants · pp. 254–265 · Published 29 Mar 2013

10.9734/EJMP/2013/1686

Abstract

Aims: We evaluated the phytochemical contents and antioxidant capacity of the methanolic leaf extract of the Nigerian Axonopus compressus. This is a preliminary investigation to determining the active principle which may be involved in the antidiabetic mechanism of the plant. Study Design: Phytochemicals and antioxidant capacity were determined using chromatographic and spectrophotometric detection methods of cold leaf extracts of Axonopus compressus. Place and Duration of Study: Department of Biochemistry, College of Natural and Applied Sciences, Michael Okpara University of Agriculture Umudike Abia State, Nigeria. Methodology: Antioxidant activities were investigated by three tests namely: 2,2-diphenyl-1-picryl hydrazyl (DPPH), Fe3+ to Fe2+ transformation (ferric reducing antioxidant power, FRAP) and a modified version of TBARS assay. These in vitro antioxidant models were carried out after cold extraction maceration. The antioxidant capacity was measured at varying concentrations (10 ~ 400 μ/ml) of the extract required to quench the free radicals by 50% (IC50) and expressed as % inhibition. Phytochemicals were determined by standard detection and spectrophotometric methods. Results: The phytochemicals: saponin (1.2±0.1), alkaloid (2.10±0.12), tannin (0.71±0.4), flavonoid (1.92±0.13) and polyphenol (1.78±0.21) in mg/100g were strongly detected. The leaf extract was found to have a concentration dependent antioxidant activity comparable with that of ascorbic acid. Axonopus compressus’s DPPH reduction was highest at 400μg/ml (92.00 ± 0.002%) with IC50 of 52.2μg/ml. The ferric reducing power of the extract at 400μg/ml (78±1.83% [FRAP:0.92])  and  the inhibition of lipid peroxidation measured as TBARS was 92.±1.21% Conclusion: The presence of these   phytochemicals and the high antioxidant power may explain the astringent action of the plant observed in its ethnomedicinal use especially in the treatment of diabetes. Our findings therefore, suggest that Axonopus compressus possess a strong antioxidant property that may substantiate its ethnomedicinal efficacy.  

Antioxidants phytochemicals flavonoid medicinal plant Axonopus compressus Nigeria

References (41)

  1. 1 Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis
  2. 2 [2] Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration [DOI]
  3. 3 Medicinal plants and traditional medicine in Africa
  4. 4 Diabetes, oxidative stress, and antioxidants: A review [DOI]
  5. 5 Phytochemical constituents of some Nigerian medicinal plants [DOI]
  6. 6 Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method [DOI]
  7. 7 Strategies of antioxidant defense [DOI]
  8. 8 Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds [DOI]
  9. 9 Assessing Antioxidant and Prooxidant Activities of Phenolic Compounds [DOI]
  10. 10 Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour [DOI]
  11. 11 Antioxidant activity of selected essential oil components in two lipid model systems [DOI]
  12. 12 Inhibition of .ALPHA.-Glucosidase and .ALPHA.-Amylase by Flavonoids [DOI]
  13. 13 Quantification of Polyphenolics and Their Antioxidant Capacity in Fresh Plums [DOI]
  14. 14 Antioxidant Activities of Some Extracts ofThymus zygis [DOI]
  15. 15 Antioxidant Phytochemicals in Fruits and Vegetables: Diet and Health Implications [DOI]
  16. 16 The Antioxidants and Pro-Antioxidants Network: An Overview [DOI]
  17. 17 A text-book of pharmacognosy
  18. 18 Polyphenolic compounds in the fruits of Egyptian medicinal plants (Terminalia bellerica, Terminalia chebula and Terminalia horrida): Characterization, quantitation and determination of antioxidant capacities [DOI]
  19. 19 Flavonoids and isoflavones: absorption, metabolism, and bioactivity [DOI]
  20. 20 Tannic Acid Stimulates Glucose Transport and Inhibits Adipocyte Differentiation in 3T3-L1 Cells [DOI]
  21. 21 Economic Botany A Textbook Of Useful Plants And Plant Products
  22. 22 Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids [DOI]
  23. 23 Phenolic composition of Flacourtia indica, Opuntia megacantha and Sclerocarya birrea [DOI]
  24. 24 Phytochemical Composition and Antioxidant Capacity of Three Malian Medicinal Plant Parts [DOI]
  25. 25 Comparative evaluation of the antioxidant potential of some Iranian medicinal plants [DOI]
  26. 26 Phenolic Components of the Plant Cell Wall [DOI]
  27. 27 Functional Foods and Health Promotion [DOI]
  28. 28 Postharvest Biochemistry of Plant Food-Materials in the Tropics
  29. 29 α-Glucosidase inhibitory effect by the flower buds of Tussilago farfara L. [DOI]
  30. 30 Tropical pasture and fodder plants.
  31. 31 In vitro antioxidant properties of Helichrysum arenarium (L.) Moench [DOI]
  32. 32 Bioactive aristolactams from Piper umbellatum [DOI]
  33. 33 Use of medicinal plants for diabetes in Trinidad and Tobago [DOI]
  34. 34 Alkaloid, tannin and saponin contents of some Nigerian medicinal plants
  35. 35 Preliminary study of antidiabetic activity of the methanolic leaf extract of Axonopus compressus (P. Beauv) in alloxan-induced diabetic rats [DOI]
  36. 36 Use of natural antioxidants to scavenge free radicals: a major cause of diseases.
  37. 37 Combined effect of total alkaloids from Feculae Bombycis and natural flavonoids on diabetes [DOI]
  38. 38 Axonopus compressus (Sw.) P. Beauv. A native grass species for phytoremediation of hydrocarbon‐contaminated soil in Assam, India [DOI]
  39. 39 Effect of Dendrophthoe falcata (L.f.) Ettingsh on female reproductive system in Wistar rats: a focus on antifertility efficacy [DOI]
  40. 40 Foliar epidermal and phytochemical studies of the grasses Cymbopogon citratus (stapf.), Axonopus compressus (P. Beauv.) and Eragrostis tremula (S. W. Beauv) in Ekpoma, Edo state, Nigeria. [DOI]
  41. 41 [Hypoglycemic effect of saponin from Tribulus terrestris].

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