Skip to content
Research Article Open access CC BY 4.0

Phytochemical Composition and in vitro Antioxidant Activity of Golden Melon (Cucumis melo L.) Seeds for Functional Food Application

Ibidapo P. Olubunmi, A. Alagbe Olajumoke, J. Awogbemi Bamidele, O. Fatunmibi Omolara

International Journal of Biochemistry Research & Review · pp. 1–13 · Published 7 Mar 2019

10.9734/ijbcrr/2019/v25i230070

Abstract

Aim: Golden melon (Cucumis melo) is an annual herbaceous plant belonging to the family of Cucurbitaceae (Cucurbit). This study was carried out to evaluate the phytochemical composition and in vitro antioxidant activity of golden melon seed extract. Place and Duration of Study: The study was carried out between a period of July and August 2017 at Baking Milling Division, Federal Institute of Industrial Research Oshodi Nigeria.  Methodology: The crude methanolic extracts of the seed were tested for phytochemical and antioxidant activities according to standard analytical procedure. The antioxidant potential of the seed extracts was examined using different assays by determining total phenolic content, total flavonoid content, total antioxidant capacity. The free radical scavenging activities of the extract such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)  radical scavenging activity,  in vitro lipid peroxidation, and nitric oxide (NO) scavenging assay were determined spectrophotometrically. Results: The phytochemical screening of the seed extracts revealed the presence of some secondary metabolites such as alkaloids, phenolic, steroids, flavonoids, terpenoids and cardiac glycosides. The total phenolic content of extract was found to be 29.39 mg/100 g while the amount of total flavonoid content was 20.67 mg/100 g. Scavenging ability was observed to increase in proportion to concentration for all the scavenging assays and at the highest concentration of 100 µg/ml. Total antioxidant capacity assay showed 19.44 mg per 100 g. This high scavenging ability in the seed extracts may be attributed to the presence of phenolic and flavonoids compounds in the extract. The DPPH free radical scavenging activity of 100µg/ml Cucumis melo extract was 75.20% ± 0.72 while the reference standard (Ascorbic acid) was 83.24% ±0.31. Lipid peroxidation inhibition ability of 100 µg/ml Cucumis melo extract was 87.18% ± 0.16 while the standard (ascorbic acid) was 94.96% ± 0.16 at the same concentration. Results obtained from this study showed that the nitric oxide scavenging ability of the extract was 80.50%±0.63 while the standard antioxidant was 85.94% ± 0.54. Conclusion: In all the assays, Cucumis melo extract showed maximum percentage of antioxidant potentials at 100 µg/ml. Additionally, golden melon seed possess appreciable amount of phenols and high antioxidant properties which could be explored and incorporated in functional food applications particularly in baked products. 

Golden melon seed phytochemical composition antioxidant activity free radical scavenging activity

References (34)

  1. 1 Structure-antioxidant activity relationships of flavonoids and phenolic acids [DOI]
  2. 2 Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E [DOI]
  3. 3 Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis
  4. 4 [1] Role of free radicals and catalytic metal ions in human disease: An overview [DOI]
  5. 5 Estimation of total flavonoid content in propolis by two complementary colometric methods [DOI]
  6. 6 Total Phenol Analysis: Automation and Comparison with Manual Methods [DOI]
  7. 7 Lipid peroxidation and antioxidants as biomarkers of tissue damage [DOI]
  8. 8 Phenolic content and antioxidant activity of olive extracts [DOI]
  9. 9 The measurement and mechanism of lipid peroxidation in biological systems [DOI]
  10. 10 Determination of Antioxidant and Antimicrobial Activities of Rumex crispus L. Extracts [DOI]
  11. 11 Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria [DOI]
  12. 12 Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas [DOI]
  13. 13 Spices as antioxidants [DOI]
  14. 14 Antioxidant Phytochemicals in Fruits and Vegetables: Diet and Health Implications [DOI]
  15. 15 Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp [DOI]
  16. 16 Glycosides in Medicine: “The Role of Glycosidic Residue in Biological Activity” [DOI]
  17. 17 Phytochemical screening of Nigerian medicinal plants II.
  18. 18 In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit [DOI]
  19. 19 Melanoidins Formed by Maillard Reaction in Food and Their Biological Activity [DOI]
  20. 20 Effect of peeling and three cooking methods on the content of selected phytochemicals in potato tubers with various colour of flesh [DOI]
  21. 21 Polyphenols in the treatment of inflammatory bowel disease and acute pancreatitis [DOI]
  22. 22 Antioxidant activity free radical-scavenging potential of Pithecellobium dulce Benth seed extracts [DOI]
  23. 23 Phytochemical and Elemental Analysis of Acalypha wilkesiana Leaf
  24. 24 In vitro Antioxidant Activities of Methanol and Aqueous Extract of Annona squamosa (L.) Fruit Pulp [DOI]
  25. 25 Nitric oxide scavenging ability of ethyl acetate fraction of methanolic leaf extracts of Chromolaena odorata (Linn.) [DOI]
  26. 26 Antiosteoporotic Agents From Natural Sources [DOI]
  27. 27 Antioxidant Activity and Pharmacological Evaluation of Cucumis melo var. agrestis Methanolic Seed Extract [DOI]
  28. 28 Antioxidant and Antibacterial Activities of Trema cannabina
  29. 29 The Morphological Characterization of the Melon Species in the Family Cucurbitaceae Juss., and their Utilization in Nigeria
  30. 30 Anti-HIV-1 protease activities of crude extracts of some Garcinia species growing in Tanzania [DOI]
  31. 31 Lipid peroxidation in the first trimester of pregnancy [DOI]
  32. 32 EFFECT OF SOY PROTEIN ISOLATE AND SOY FIBER ON COLOR, PHYSICAL AND SENSORY CHARACTERISTICS OF BAKED PRODUCTS [DOI]
  33. 33 Antioxidant, Antidiarrhoeal and Cytotoxic Properties of Punica granatum Linn.
  34. 34 [Polyphenols and endogenous antioxidant defences: effects on glutathione and glutathione related enzymes].

Cited by 32

Anti-atherogenic and cardio-protective properties of sweet melon (Cucumis melo. L. Inodorus) seed extract on high fat diet induced obesity in male wistar rats

G. Adebayo-Gege, V. Alicha, T. O. Omayone · BMC Complementary Medicine and Therapies · 2022

Molecular docking and anti-ulcerative potential of Cucumis (L. Inodorous) on ibuprofen induced gastric ulceration in male wistar animals

Grace Adebayo-Gege, Zainab Shehu Uthman, Moses Dele Adams · Biomedicine & Pharmacotherapy · 2023

Comprehensive Review on Fruit Seeds: Nutritional, Phytochemical, Nanotechnology, Toxicity, Food Biochemistry, and Biotechnology Perspective

Sarita Roy, Tanmay Sarkar, Vijay Jagdish Upadhye · Applied Biochemistry and Biotechnology · 2023

Showing 13 of 32 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

47

Page views

0

PDF downloads

4

Outbound clicks

32

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.