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Phytochemical, Antioxidant and Antimicrobial Potentials of Azadirachta indica Leaf Extract Using Bacteria Isolated from Polluted Water as Test Organisms

Olusola Nathaniel Majolagbe, Bolatito Motunrayo Ishola, Ezekiel Gbadebo Adeyeni

Journal of Advances in Biology & Biotechnology · pp. 24–34 · Published 14 Jul 2023

10.9734/jabb/2023/v26i5634

Abstract

Aims: Globally, there is continuous emergence of infectious pathogens. Therefore, there is a need to search for new drug molecules of plant origin such as A. indica which is commonly grown in Nigeria. This study was aimed at evaluating the antioxidant and antimicrobial potentials of the leaf extract of A. indica and determining its bioactive molecules. Study Design: This was an in-vitro laboratory study. Place and Duration of Study: This research was carried out at the Microbial Resources Research Laboratory (MRRL), Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso Nigeria from April 2021 to January 2023. Methodology: Collected fresh leaves of A. indica were destalked, air dried at 27oC for 10 days, and finally grounded. The dried powders were extracted by maceration using four (4) solvents namely: water (aqueous), methanol, ethyl-acetate, and n-hexane. The leaf extracts were assessed for their antioxidant and antimicrobial potential. Bioactive molecules present in the leaf extract were determined by Gas chromatography-mass spectrometry. Results: It was observed that leaf extracts of A. indica possess bioactive molecules such as Phytol which is over 50% of the total biomolecules present. These biomolecules are responsible for the antimicrobial and antioxidant potentials of the extract. The ABTS and DPPH radicals scavenging activities ranged from 24.51 to 90.32 % and 31.79 to 79.59 % respectively. These extracts can be used to mop up free radicals which have damaging effects on the cells of the body. The minimum inhibitory concentration (MIC) of the extract for each of the test bacteria isolates varied between 0.05 to 0.8 mg/ml. Thus, the leaf extract of A. indica is a reservoir of bioactive molecules which can be used for novel drug synthesis and discovery. Conclusion: Thus, leaf extracts of A. indica contain potent biomolecules which can be harnessed for therapeutic use.

Azadirachta indica leaf extract antimicrobial antioxidant biomolecules drug synthesis

References (21)

  1. 1 Use of a free radical method to evaluate antioxidant activity [DOI]
  2. 2 Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria [DOI]
  3. 3 Medicinal Plants and Traditional Medicine in Africa [DOI]
  4. 4 Biological activities and medicinal properties of neem (Azadirachta indica)
  5. 5 Anticancer polysaccharides from natural resources: A review of recent research [DOI]
  6. 6 Complex Interactions between Phytochemicals. The Multi-Target Therapeutic Concept of Phytotherapy [DOI]
  7. 7 Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees [DOI]
  8. 8 Bacterial resistance to antibiotics: Active efflux and reduced uptake [DOI]
  9. 9 Screening of some Palestinian medicinal plants for antibacterial activity [DOI]
  10. 10 Evaluation of the chemical composition of two Nigerian medicinal plants
  11. 11 Neem—An Omnipotent Plant: A Retrospection [DOI]
  12. 12 Anti-fungal and anti-bacterial activity of some herbal remedies from Tanzania [DOI]
  13. 13 Clinical studies on the effect of Neem (Azadirachta indica) bark extract on gastric secretion and gastroduodenal ulcer [DOI]
  14. 14 Potential antimalarials from African natural products: a review [DOI]
  15. 15 Evaluation of the antibacterial potential of some plants against human pathogenic bacteria
  16. 16 ANTIMICROBIAL ACTIVITY OF AQUEOUS EXTRACT OF Terminalia chebula RETZ. ON GRAMPOSITIVE AND GRAMNEGATIVE MICROORGANISMS
  17. 17 Antibacterial Activity of Calycophyllum spruceanum Leaf Extract against Enterococcus faecalis Strains “In vitro” for Endodontic Purposes [DOI]
  18. 18 ANTI-MICROBIAL ACTIVITY OF NEEM OIL
  19. 19 The effect of drying time on the yield and the chemical composition of essential oil and dissolved oil in hydrolat from aerial parts of Moroccan Thymbra capitata (L.) Cav [DOI]
  20. 20 Anti fungal activity of essential oils of three medicinal plants
  21. 21 Various Uses of Neem Products [DOI]

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