Phytochemical Analysis and In-vitro Antitrypanosomal Activity of Selected Medicinal Plants in Niger State, Nigeria
F. M. Madaki, A. Y. Kabiru, A. Mann, A. Abdulkadir, J. N. Agadi, A. O. Akinyode
International Journal of Biochemistry Research & Review · pp. 1–7 · Published 25 Mar 2016
10.9734/IJBCRR/2016/24955Abstract
Trypanosomiasis is a disease caused by protozoan parasite belonging to the genus Trypanosoma. The disease affects both humans and animals. In this study, phytochemical analysis and in-vitro antitrypanosomal screening of crude methanol extracts of the leaves of Waltheria indica, Vernonia amygdalina, Albizia ferruginea, Camellia sinensis, Chamaecrista mimosoides and Hyptis suaveolens were carried out using standard methods. Highly parasitized blood from infected donor rats was diluted with glucose phosphate buffered saline solution and incubated with varying concentrations (1 mg/ml, 2 mg/ml and 4 mg/ml) of the extracts in Eppendorf tubes for 60 minutes. Aliquots from the mixtures were removed and observed under microscope for parasite motility at 5 minutes interval. Phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, phenols and glycosides in all the plants’ extracts. Saponins were absent in H. suaveolens while steroids and phlobatannins were absence in C. mimosoides and H. suaveolens. All the plants’ extracts showed significant cessation of parasite motility with increase in incubation time and concentration of the extract. Complete cessation of the parasite motility was observed for all the extracts within 60 minutes of the study. The most active extract was W. indica at 4mg/ml which caused complete cessation of the parasite motility within 5 minutes whereas the least active plants C. mimosoides and H. suaveolens. Both caused complete cessation of the parasite motility within 25 minutes. Berenil, the standard drug, however, caused cessation of trypanosomal motility within 5 minutes even at 1 mg/ml. These results showed that methanol l extracts of the plants leaves screened especially Waltheria indica and Vernonia amygdalina have significant in vitro antitrypanosomal activity and may be potential source for the in vivo treatment of trypanosomiasis.
References (8)
- 1 Phytochemical screening of Nigerian medicinal plants II.
- 2 Antioxidative and Chemopreventive Properties of Vernonia amygdalina and Garcinia biflavonoid [DOI]
- 3 The Human African Trypanosomiasis Control and Surveillance Programme of the World Health Organization 2000–2009: The Way Forward [DOI]
- 4 Evaluation of chemical composition of indeginous species and flavouring agents [DOI]
- 5 Interactions of condensed tannins with selected proteins [DOI]
- 6 Antiamoebic activity of Piper longum fruits against Entamoeba histolytica in vitro and in vivo [DOI]
- 7 Effects of methanol extract of Vernonia amygdalina leaf on survival and some biochemical parameters in acute Trypanosoma brucei brucei infection
- 8 Studies on the In Vitro Trypanocidal Effect of the Extracts of Some Selected Medicinal Plants in Sokoto State, Nigeria [DOI]
Cited by 14
Domitila Villalba, Melissa Escobar, Cinthia Cazal Martínez · Journal of Applied Pharmaceutical Science · 2025
Dickson Achimugu Musa, Spencer Chibueze Asogwa, Egbenoma Andrew Aigboeghian · Trends in Pharmacology and Toxicology · 2025
Afisu Basiru, Ganiu Jimoh Akorede, Kehinde Soetan · Journal of Complementary and Integrative Medicine · 2019
M.O., Tom-Otu, O. S. Omowaye, D. Makolo · Nigerian Journal of Pure and Applied Sciences · 2023
Lawal, S. M., Inabo, H. I., Ella, E. E. · UMYU Journal of Microbiology Research (UJMR) · 2024
Deborah Adebukola Oloruntola, Ebenezer Oluyemi Dada, Muftau Kolawole Oladunmoye · Open Veterinary Science · 2021
Showing 6 of 14 known citations — external sources report more than can currently be individually listed.
Related research
- Changes in Liver Histomorphology, Hematological Parameters and Lipid Profile of Cadmium-Exposed Rats Treated with Combined Leaf Extract of Vernonia amygdalina and Occimum gratissimum — shares topic coverage
- Phytochemical and Antibacterial Investigation of Leaf Extracts of Vernonia amygdalina — shares topic coverage
- Antimicrobial Activity of Vernonia amygdalina Leaves Aqeuous Extracts against Multidru-resistant Bacterial Isolates from Poultry Chickens in Bauchi Metropolis — shares topic coverage
- Evaluation of the Effects of the Extract of Vernonia amygdalina on Fungi Associated with Infected Tomatoes (Lycopersicon esculentum) in Jos North Local Government Area, Plateau State, Nigeria — shares topic coverage
- Microbial Safety, Antioxidant Capacity, and Physicochemical Changes in Pasteurized Mint- and Vernonia amygdalina-Flavored Pineapple-Cucumber Juice Blend Stored at Ambient Temperature — shares topic coverage
Article metrics
Real usage data collected on this platform.
71
Page views
0
PDF downloads
37
Outbound clicks
14
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.