Antimicrobial Efficacy of Silver Nanoparticles (AgNPs) in Combination with Hand Sanitizer against Foodborne  and Spoilage Bacteria

Neha Chatterjee; Akshada Shankar Ganesh; Arjya Roy; Aniesha Owlak

doi:10.9734/mrji/2026/v36i41735

Research Article Open access CC BY 4.0

Antimicrobial Efficacy of Silver Nanoparticles (AgNPs) in Combination with Hand Sanitizer against Foodborne and Spoilage Bacteria

Neha Chatterjee, Akshada Shankar Ganesh, Arjya Roy, Aniesha Owlak

Microbiology Research Journal International · pp. 130–135 · Published 13 Apr 2026

10.9734/mrji/2026/v36i41735

Abstract

Silver nanoparticles (AgNPs) are recognized for their broad-spectrum antimicrobial properties against foodborne bacteria, including E. coli, S. aureus, and B. subtilis. This study investigated whether AgNPs at varying concentrations (0.10%, 0.25%, 0.50%) could enhance the antimicrobial efficacy of a diluted hand sanitizer (1%) against these three strains. Bacterial growth was monitored by optical density (OD600) at 2, 4, and 24 hours post-inoculation. UV-Visible spectroscopy confirmed AgNP synthesis (SPR peak at 430 nm). The optimum AgNP concentration was organism-dependent: 0.25% AgNPs with 1% sanitizer produced greatest inhibition of E. coli and B. subtilis at 4 hours, while 0.50% AgNPs showed superior and sustained inhibition of S. aureus at 4 and 24 hours. These differences are attributed to cell wall architecture. AgNP-enhanced sanitizers show potential for food safety applications, though cytotoxicity concerns require resolution before human use.

Silver nanoparticles AgNPs, antimicrobial activity hand sanitizer optical density E. coli S. aureus B. subtilis

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Neha Chatterjee, Akshada Shankar Ganesh, Arjya Roy, Aniesha Owlak (2026). Antimicrobial Efficacy of Silver Nanoparticles (AgNPs) in Combination with Hand Sanitizer against Foodborne and Spoilage Bacteria. Microbiology Research Journal International , 130–135. https://doi.org/10.9734/mrji/2026/v36i41735

Affiliations

Neha Chatterjee — School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
Akshada Shankar Ganesh — School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
Arjya Roy — School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
Aniesha Owlak — School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.

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