Skip to content
Research Article Open access CC BY 4.0

PCR and Sequencing Base Detection of Gummosis Disease on Citrus aurantifolia Caused by Lasiodiplodia theobromae and Evaluation of Its Antagonisms

Md. Faruk Hasan, Md. Asadul Islam, Biswanath Sikdar

Journal of Advances in Microbiology · pp. 77–90 · Published 2 Apr 2020

10.9734/jamb/2020/v20i330230

Abstract

Diplodia gummosis disease caused by Lasiodiplodia theobromae is an economically important postharvest fruit decay that occurs on all types of citrus grown in Bangladesh. The present investigation was conducted to isolate and identify the pathogenic fungus responsible for postharvest diplodia gummosis disease (DGD) of citrus as well as the evaluation of its biological control through microbial antagonists. DGD causing pathogen was identified by physiological, morphological and molecular methods. The pathogenic fungus was isolated from the surface of postharvest lime fruits. The optimum growth of the fungi was observed in maltose, 2% NaCl, 15% sugar and pH 7 containing PDA medium at 35⁰C. PCR products of the internal transcribed spacer (ITS) region of the fungus showed approximately 650 bp size clear band in gel electrophoresis. The amplified region of the fungus showed 99.62% similarities with the sequences of L. theobromae. Artificially inoculation of the fungus in malta, musumbi, sweet orange, lime, and guava fruits showed similar size clear band and typical diplodia gummosis disease symptom. Methanol extracts of Datura metal displayed the highest inhibition (75.25%) against the isolated fungus. Non-pathogenic fungi Trichoderma viride showed the highest antagonistic efficiency followed by Neofusicoccum mangifera against the isolated fungus. The tested soil bacteria did not show significant antagonistic activity against the isolated fungus. Therefore, the DGD of citrus control system should be integrated into the overall citrus postharvest decay control system to reduce all citrus postharvest diseases and to protect fresh citrus fruit values.

Citrus aurantifolia PCR sequencing Lasiodiplodia theobromae; antagonisms.

References (22)

  1. 1 AMPLIFICATION AND DIRECT SEQUENCING OF FUNGAL RIBOSOMAL RNA GENES FOR PHYLOGENETICS [DOI]
  2. 2 The draft genome of sweet orange (Citrus sinensis) [DOI]
  3. 3 Citrus fruits as a treasure trove of active natural metabolites that potentially provide benefits for human health [DOI]
  4. 4 Candida albicans drug resistance – another way to cope with stress [DOI]
  5. 5 Frequency of Citrus Fruit Intake Is Associated With the Incidence of Cardiovascular Disease: The Jichi Medical School Cohort Study [DOI]
  6. 6 Diversity and host association of the tropical tree endophyte Lasiodiplodia theobromae revealed using simple sequence repeat markers [DOI]
  7. 7 In vitro Antimicrobial Activity of Citrus aurantifolia and its Phytochemical screening [DOI]
  8. 8 Identification and pathogenicity of Lasiodiplodia theobromae causing dieback of table grapes in Peru [DOI]
  9. 9 Antifungal activity of some plant extracts on some pathogenic fungi [DOI]
  10. 10 The rot fungus Botryodiplodia theobromae strains cross infect cocoa, mango, banana and yam with significant tissue damage and economic losses [DOI]
  11. 11 SCREENING OF ANTIMICROBIAL, CYTOTOXIC AND PESTICIDAL ACTIVITIES OF COCCINIA GRANDIS (L.) VOIGT [DOI]
  12. 12 Circumscription of Botryosphaeria Species Associated with Proteaceae Based on Morphology and DNA Sequence Data [DOI]
  13. 13 In Vitro Antifungal Activity of Citrus aurantifolia Linn Plant Extracts against Phytopathogenic Fungi Macrophomina phaseolina [DOI]
  14. 14 Isolation and characterization of a canker disease causing pathogen from Citrus aurantifolia and evaluation of its biological control measure
  15. 15 Morphological and Molecular Variability of Lasiodiplodia theobromae Causing Stem End Rot of Mango in Tamil Nadu, India [DOI]
  16. 16 Characterization of Crown Rot Disease of Banana Fruit and Eco-Friendly Quality Improvement Approach during Storage [DOI]
  17. 17 Characterization of fungal pathogens causing diseases in bitter gourd and establishment of their eco-friendly control measure
  18. 18 Effects of a penthiopyrad and picoxystrobin fungicide mixture on phoma stem canker (Leptosphaeria spp.) on UK winter oilseed rape [DOI]
  19. 19 Phytochemical Content and In Vitro Antimycelial Efficacy of Colocasia esculenta (L), Manihot esculenta (Crantz) and Dioscorea rotundata (Poir) Leaf Extracts on Aspergillus niger and Botryodiplodia theobromae [DOI]
  20. 20 Citrus Flavonoids with Skin Lightening Effects - Safety and Efficacy Studies
  21. 21 Effect of postharvest treatments on quality aspect of Maghrabi banana fruit.
  22. 22 IDENTIFICATION AND CHARACTERIZATION OF CAUSAL AGENT OF CERCOSPORA LEAF SPOT DISEASE OF OKRA [DOI]

Cited by 20

Mycobiota associated with “Grapevine Trunk Disease” in San Juan, Argentina

Melisa Belén Rosa Manzano, Mario Rajchenberg, Lucía Molina · Journal of Plant Diseases and Protection · 2025

Morphomolecular and cultural characteristics and host range of Lasiodiplodia theobromae causing stem canker disease in dragon fruit

Preangka S. Briste, Abdul M. Akanda, Md. Abdullahil B. Bhuiyan · Journal of Basic Microbiology · 2022

Morphological, Physiological, Biochemical, and Molecular Characterization of Fungal Species Associated with Papaya Rot in Cameroon

Moussango Victor Davy, Voundi Olugu Steve, Tchabong Raymond Sammuel · Journal of Fungi · 2025

First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh

Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath Sikdar · F1000Research · 2020

Citrus stem rot disease (Lasiodiplodia theobromae (Pat.) Griff. & Maubl) problem and their control strategy in Indonesia

M E Dwiastuti, T G Aji · IOP Conference Series: Earth and Environmental Science · 2021

Evaluation of possible biological control of Fusarium sp. using plant extracts and antagonistic species of microbes in vitro

Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath Sikdar · F1000Research · 2020

First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh

Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath Sikdar · F1000Research · 2023

Showing 9 of 20 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

37

Page views

0

PDF downloads

7

Outbound clicks

20

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.