Testicular Toxicity Cum Actions of Moringa oleifera Seeds Extract Following Nutmeg Administration

Abstract

This study was carried out to assess the effect of Moringa oleifera seed extracts on nutmeg induced toxicity on the testes of adult male Albino Wistar rats. After two weeks of acclimatization, the rats were divided into ten equal groups designated as A, B, C, D, E1, E2, F1, F2, G1, and G2; each group consisted of three rats. Group A (control group) were administered distilled water, Group B were orally administered 0.70 ml of nutmeg, Group C were orally administered 1.40 ml of nutmeg, Group D were administered 10g of nutmeg via inhalation. Group E1 were orally administered 0.70 ml of nutmeg for four weeks, followed with 1.5 ml of ethanolic extract of Moringa seed on the fourth week, Group E2 were orally administered 0.70 ml of nutmeg for four weeks, followed with 1.3 ml of n-hexane extract of Moringa seed on the fourth week, Group F1 were orally administered 1.40 ml of nutmeg for four weeks, followed by 3.0 ml of ethanolic extract of Moringa seed on the fourth week, Group F2 were orally administered 1.50 ml of nutmeg for four weeks, followed by 3.0 ml of n-hexane extract of Moringa seed on the fourth week, Group G1 were administered 10g of nutmeg via inhalation for four weeks, followed by oral administration of 2.2 ml of ethanolic extract of Moringa seed on the fourth week and Group G2 animals were administered 10g of nutmeg via inhalation for four weeks, followed by oral administration of 3.0 ml of n-hexane extract of Moringa seed on the fourth week. On the 28th day, the rats were anaesthetized via chloroform inhalation, sacrificed and the testes were harvested and fixed immediately in 10% buffered formalin, processed and stained with Harris Haematoxylin and Eosin (H&E) staining method. Data were expressed as Mean ± standard error of the Mean (M±SEM) and subjected to one way analysis of variance (ANOVA). Significant difference between mean was accessed by student-New-Man-Keuls post hoc test. 95% level of significance (p < 0.05) was used for statistical analysis. At the end of the experiment Moringa oleifera seed extracts showed ameliorative and protective functions from the toxicity of excess nutmeg on the testes.