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Research Article Open access CC BY 4.0

Partial Purification of a Catalase from an Improved Nigerian Sorghum Grain Variety

C. I. Nnamchi, B. C. Nwanguma, O. C. Amadi

Asian Journal of Chemical Sciences · pp. 30–37 · Published 7 Nov 2020

10.9734/ajocs/2020/v8i419051

Abstract

Catalases are key components of cellular detoxification pathways that prevent the formation of highly reactive hydroxyl radicals through catalyzing the decomposition of hydrogen peroxide into water and molecular oxygen. Their presence in brewery grains prevent the inactivation of important brewery enzymes and also stop lipid peroxidation. To determine their occurrence and establish some of its properties in sorghum, which has become as an important brewery grain similar to barley, crude catalase was obtained from a sorghum grain variety. Preliminary purification of catalase from the sorghum grain variety used, NRL-3, showed that the enzyme was purified 3.2-fold from the crude protein to give a 49% yield of the partially purified enzyme, with a final specific activity of 32 Umg-1 proteins. There was also a positive indication of sorghum catalase presence on SDS PAGE with positive bands occurring between the range of 48-62 kDa. Therefore, the molecular weight of sorghum catalase most likely falls within the two bands. The enzyme showed a narrow pH range with optimum activity occurring at pH 7. Similarly, its optimum activity temperature occurred at 40°C.  This work is the first reported attempt at purifying catalase from sorghum.

Catalase sorghum cereals partial purification antioxidant reactive oxygen species.

References (21)

  1. 1 A Large Family of Class III Plant Peroxidases [DOI]
  2. 2 Tropical Crops: Monocotyledons [DOI]
  3. 3 Novel food and non-food uses for sorghum and millets [DOI]
  4. 4 Regulation and measurement of oxidative stress in apoptosis [DOI]
  5. 5 Free Radicals in Biology and Medicine 4th ed.
  6. 6 Diversity of properties among catalases [DOI]
  7. 7 UVB Light Stimulates Production of Reactive Oxygen Species [DOI]
  8. 8 Current perspectives on the role of enzymes in brewing [DOI]
  9. 9 Digestibility of protein and starch from sorghum (Sorghum bicolor) is linked to biochemical and structural features of grain endosperm [DOI]
  10. 10 Oxidation of Catalase by Singlet Oxygen [DOI]
  11. 11 Peroxidases and catalases : biochemistry, biophysics, biotechnology, and physiology
  12. 12 Marker-Assisted Selection in Sorghum [DOI]
  13. 13 Expression of the developmentally regulated catalase (cat) genes in maize [DOI]
  14. 14 Hydrogen peroxide scavenging regulates germination ability during wheat (Triticum aestivumL.) seed maturation [DOI]
  15. 15 OXYGEN-SCAVENGING ENZYMES IN BARLEY AND MALT AND THEIR EFFECTS DURING MASHING [DOI]
  16. 16 ISOENZYME PROFILES OF PEROXIDASE, CATALASE AND SUPEROXIDE DISMUTASE AS AFFECTED BY DEHYDRATION STRESS AND ABA DURING GERMINATION OF WHEAT SEEDS
  17. 17 Structural and spectroscopic characterisation of a heme peroxidase from sorghum [DOI]
  18. 18 Purification and Properties of Catalase from Van Apple (Golden Delicious) [DOI]
  19. 19 Partial Purification and Properties of Catalase from Brassia oleracea capitata [DOI]
  20. 20 Purification and Properties of Catalase from Sweet Potato Root Microbodies [DOI]
  21. 21 HEAT SENSITIVITY, OPTIMUM pH AND CHANGES IN ACTIVITY OF SORGHUM PEROXIDASE DURING MALTING AND MASHING [DOI]

Cited by 2

Peroxidase, an Example of Enzymes with Numerous Applications

I. Nnamchi, Chukwudi, C. Amadi Onyetugo, I. Nnaji Amarachi · Asian Journal of Chemical Sciences · 2021

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