Genetic Analysis of (Oreochromis niloticus) Nirvana Tilapia Cultivated in Wanayasa and Galunggung, Lumajang, Bali, Minahasa Using Random Amplified Polymorphic DNA Method
Ayi Yustiati, Ibnu Bangkit Bioshina Suryadi, . Rosidah, Walim Lili, Dian Yuni Pratiwi, Anandita Rahmania, Maryam Nur Latifah
Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 1–7 · Published 22 Sep 2022
10.9734/ajbgmb/2022/v12i330292Abstract
This research aims to determine the kinship of nirvana tilapia (Oreochromis niloticus) cultivated in Wanayasa with Galunggung, Lumajang, Bali, and Minahasa by Random Amplified Polymorphic DNA method. All tilapia cultivated in these four places originated from Wanayasa. This research was conducted in the Biotechnology Laboratory, Laboratory of Microbiology and Molecular Biotechnology, FPIK UNPAD. The research method was an explorative experiment with the RAPD-PCR technique using primary OPA-3 and OPA-5. However, the results of DNA amplification with OPA-3 were better than with OPA-5. Therefore, further analysis was carried out using data from OPA-3. Kinship was analyzed based on the similarity index which was calculated by Numerical Taxonomy and Multivariate Analysis System (NTSYS) program. The results showed that the kinship between Nirvana tilapia cultivated in Wanayasa with Galunggung, Lumajang, Bali, and Minahasa, according to the similarity index was high, about 77-85%. The highest similarity index (85%) was obtained between nirvana tilapia cultivated in Wanayasa with Galunggung, and the lowest similarity index (77%) between tilapia cultivated in Bali with Minahasa. Genetic changes that occur because of differences in cultivation environments range from 15-23%.
Cited by 0
No indexed citations yet.
Related research
- The Effect of E-waste Dumpsite Soil on Cultured Tilapia Fish and Associated Microorganisms — shares topic coverage
- Isolation and Characterization of Fungal Strains from Tilapia (Oreochromis niloticus) and Machoiron (Chrysichthys nigrodigitatus) Fish in Taabo Lake, Kossou Lake, and Tagba Lagoon of Grand Lahou, Côte d’Ivoire — shares topic coverage
- Effects of Different Drying Methods on Quality Parameters of Tilapia (Oreochromis niloticus) Obtained from the Local Market of Bangladesh — shares topic coverage
- Tilapia – An Excellent Candidate Species for World Aquaculture: A Review — shares topic coverage
- The Effect of High Temperature on the Growth Performance of Hybrid Tilapia Oreochromis niloticus X Oreochromis aureus Juveniles Reared in a Recycling System — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.