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Research Article Open access CC BY 3.0

Spectral Analysis and Chemical Studies of the Sweet Constituent, Rebaudioside A

Venkata Sai Prakash Chaturvedula, Indra Prakash

European Journal of Medicinal Plants · pp. 57–65 · Published 8 Feb 2012

10.9734/EJMP/2012/1009

Abstract

Aim: To study NMR spectral analysis and hydrolysis products of Rebaudioside A (1) that has been isolated from Stevia rebaudiana. Study Design: Acquiring NMR and high resolution mass spectral (HRMS) data of Rebaudioside A for structural characterization, and identification of hydrolysis products. Place of Study: Organic Chemistry Department of The Coca-Cola Company, Atlanta, GA 30313, USA. Methodology: The 1D (1H &13C) and 2D (COSY, HSQC, and HMBC) NMR spectral data of was acquired using Varian Unity Plus 600 MHz instrument using standard pulse sequences.Hydrolysis studies were performed on Rebaudioside A using acid, base and enzymatic studies to identify partial degradation products, aglycone and sugar residues. Results: The proton and carbon values of Rebaudioside A were assigned based on NMR and HRMS data as well as chemical studies. The aglycone was identified as steviol on the basis of enzymatic hydrolysis, sugar was identified as D-glucose from acid hydrolysis and the partial hydrolyzed product was identified as Rebaudioside B from alkaline hydrolysis.

Stevia rebaudiana compositae asteraceae Rebaudioside A spectral data TLC comparison hydrolysis studies.

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