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Research Article Open access CC BY 4.0

Optimization of Spirulina’s Phycocyanin Extraction Yield Using Response Surface Method

Truong Phan Nhat, Mau Trinh-Dang

Biotechnology Journal International · pp. 52–59 · Published 7 Apr 2023

10.9734/bji/2023/v27i1673

Abstract

Besides cultivation, extraction is also a critical stage in enhancing the yield of phycocyanin production - a highly valuable compound from Spirulina biomass. In this study, the combined effect of three important variables in the ultrasonic-assisted extraction process on phycocyanin extraction yield, namely extraction temperature, sonication time, and solvent pH were investigated through a central composite design experiment. Furthermore, the response surface method was applied in order to define an optimal condition to achieve the highest extraction yield. The results showed that when temperature ranged from 35ºC to 45ºC, sonication time from 20 to 50 minutes, and solvent pH from 6 to 8, the average yield of 30.135±1.552 mg/g was obtained with an average purity of 0.871±0.043. A regression model was also successfully developed, which allowed a good prediction of extraction yield based on the three mentioned variables. On the other hand, an optimal condition for extraction was also proposed with sonication time = 43.57 minutes, extraction temperature = 37.6oC, and solvent pH = 6.7. These results were practically valuable for the improvement of phycocyanin extraction from Spirulina biomass.

Phycocyanin response surface method Arthrospira platensis ultrasound-assisted extraction

References (31)

  1. 1 COMPLEMENTARY CHROMATIC ADAPTATION IN A FILAMENTOUS BLUE-GREEN ALGA [DOI]
  2. 2 Response-Surface Methods in R , Using rsm [DOI]
  3. 3 Phycocyanin from Spirulina sp: influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyanin [DOI]
  4. 4 Optimization of olive leaf extract obtained by ultrasound-assisted extraction with response surface methodology [DOI]
  5. 5 Stability of phycocyanin extracted from Spirulina sp.: Influence of temperature, pH and preservatives [DOI]
  6. 6 Optimization of phycocyanin extraction from Spirulina platensis using factorial design [DOI]
  7. 7 Recent Developments in Production and Biotechnological Applications of C-Phycocyanin [DOI]
  8. 8 Heat and light stability of three natural blue colorants for use in confectionery and beverages [DOI]
  9. 9 Thermal stability improvement of blue colorant C-Phycocyanin from Spirulina platensis for food industry applications [DOI]
  10. 10 Utilization of Response Surface Methodology in Optimization of Extraction of Plant Materials [DOI]
  11. 11 Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540) [DOI]
  12. 12 Optimization of ultrasound assisted extraction of bioactive components from brown seaweed Ascophyllum nodosum using response surface methodology [DOI]
  13. 13 Effect of ultrasonic treatment on the polyphenol content and antioxidant capacity of extract from defatted hemp, flax and canola seed cakes [DOI]
  14. 14 C-phycocyanin extraction from Spirulina platensis wet biomass [DOI]
  15. 15 Extraction and purification of phycocyanin from Calothrix sp. [DOI]
  16. 16 Effects of various factors of ultrasonic treatment on the extraction yield of all-trans-lycopene from red grapefruit (Citrus paradise Macf.) [DOI]
  17. 17 Simple and efficient method for extraction of C-Phycocyanin from dry biomass of Arthospira platensis [DOI]
  18. 18 Thermal degradation kinetics of the phycocyanin from Spirulina platensis [DOI]
  19. 19 A novel method of single step hydrophobic interaction chromatography for the purification of phycocyanin from Phormidium fragile and its characterization for antioxidant property [DOI]
  20. 20 Optimization of Ultrasound-Assisted Extraction of Anthocyanins from Mulberry, Using Response Surface Methodology [DOI]
  21. 21 Protective effect of intracellular ice during freezing? [DOI]
  22. 22 Potentials and applications of algal pigments in biology and industry [DOI]
  23. 23 Response Surface Optimization of Ultrasound Assisted Extraction (UAE) of Phycocyanin from Microalgae Spirulina platensis [DOI]
  24. 24 Ammonium chloride: a novel effective and inexpensive salt solution for phycocyanin extraction from Arthrospira (Spirulina) platensis [DOI]
  25. 25 Optimization of C-phycocyanin production from S. platensis cultivated on mixotrophic condition by using response surface methodology [DOI]
  26. 26 An effective method for extraction of high purity phycocyanins (C-PC and A-PC) from dry biomass of Arthrospira maxima [DOI]
  27. 27 Extraction, identification, and quantitation of phycobiliprotein pigments from phototrophic plankton [DOI]
  28. 28 Production of caffeinated and decaffeinated green tea catechin powders from underutilised old tea leaves [DOI]
  29. 29 Phycocyanin extraction from Spirulina platensis and extract stability under various pH and temperature.
  30. 30 The effects of temperature and frequencies in ultrasound assisted extraction of phycocyanin from microalgae Spirulina sp [DOI]
  31. 31 R: A Language and Environment for Statistical Computing [DOI]

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