Skip to content
Research Article Open access CC BY 4.0

Optimization of Polyphenols and Carotenoids Extraction from Leaves of Cassia auriculata for Natural Health Products

K. D. P. P. Gunathilake, K. K. D. S. Ranaweera, H. P. V. Rupasinghe

Asian Plant Research Journal · pp. 14–25 · Published 22 Aug 2020

10.9734/aprj/2020/v6i130118

Abstract

Aims: C. auriculata leaves contains polyphenols and carotenoids and also it posses various antioxidant activities towards free radical scavenging, lipid peroxidation inhibition and reducing potential. The present study investigated the optimization of polyphenols and carotenoids extraction from Cassia auriculata leaves by response surface methodology (RSM). Study Design: A three-factor, three-levels central composite design (CCD) was performed to determine the effect of solvent concentration (30-100%), extraction temperature (30-60°C) and extraction time (30-90 min) to obtain the best extraction parameters. Place and Duration of Study: Fresh C. auriculata leaves were collected from home gardens in Makandura area of Sri Lanka and the experiments were conducted at the Department of Food Science and Technology of Wayamba University of Sri Lanka between June 2016 and August 2016. Methodology: Total polyphenol and carotenoid contents of the ethanolic extracts of the C. auriculata leaves were determined. Total polyphenols and carotenoids content in the extracts were used as the response variables.  According to the design used, twenty randomized experiments including six replicates as the center points were assigned based on the combinations of extraction variables used CCD and the values of independent process variables considered, as well as response variables. The optimal value of these factors was determined suing response surface methodology. Predicted values were compared with experimental values. Results: The optimum extraction conditions for phenolics and carotenoids were 45.4% ethanol; 19.8°C; 110.5 min and 100% ethanol; 70.2°C; 9.5 min respectively. The optimal predicted contents for total polyphenols and carotenoids were 13.08 mg GAE/g-DW and 17.31 mg/g-DW respectively. Validation experiments results had good agreement with the predicted responses by RSM. Conclusion: Ethanol concentration was the most significant factor affecting on total polyphenols and carotenoids extraction. Extraction temperatures and time did not significantly influence on carotenoids and polyphenols extraction from leaves of C. auriculata.  The estimated optimum extraction conditions; were established and they were very close to the experimental values. These parameters can be used as the guidelines for scale-up extraction of bioactives from the leaves of C. auriculata.

Cassia auriculata leaves polyphenols carotenoids ethanol response surface methodology

References (19)

  1. 1 Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents [DOI]
  2. 2 β-Carotene and Other Carotenoids as Antioxidants [DOI]
  3. 3 Optimization of extraction of phenolic compounds from wheat using response surface methodology [DOI]
  4. 4 Polyphenols and cardiovascular disease: effects on endothelial and platelet function [DOI]
  5. 5 Spectrophotometric determination of chlorophyll-A,B and total carotenoid contents of some algae species using different solvents
  6. 6 The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts [DOI]
  7. 7 Relationship between the colour and the chemical structure of carotenoid pigments [DOI]
  8. 8 Optimisation of the solvent extraction of bioactive compounds from Parkia speciosa pod using response surface methodology [DOI]
  9. 9 Effect of extraction conditions on lycopene extractions from tomato processing waste skin using response surface methodology [DOI]
  10. 10 Ultrasound assisted extraction of total phenolics from Cassia auriculata leaves and evaluation of its antioxidant activities [DOI]
  11. 11 Influence of Boiling, Steaming and Frying of Selected Leafy Vegetables on the In Vitro Anti-inflammation Associated Biological Activities [DOI]
  12. 12 Antioxidative properties of 34 green leafy vegetables [DOI]
  13. 13 Antioxidant compounds from a South Asian beverage and medicinal plant, Cassia auriculata [DOI]
  14. 14 Response surface optimization for recovery of polyphenols and carotenoids from leaves of Centella asiatica using an ethanol‐based solvent system [DOI]
  15. 15 Response surface optimisation for the extraction of phenolics and flavonoids from a pink guava puree industrial by‐product [DOI]
  16. 16 Response surface optimization of ultrasonic‐assisted extraction of carotenoids from oil palm ( E laeis guineensisJacq.) fronds [DOI]
  17. 17 Chemical Structures and Hepatoprotective Effects of Constituents from Cassia auriculata Leaves [DOI]
  18. 18 Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology [DOI]
  19. 19 Optimization of Phenolics and Carotenoids Extraction from Leaves of Passiflora edulis Using Response Surface Methods [DOI]

Cited by 3

<i>Cassia auriculata</i> L.–A mini review of phytochemical compounds and their antidiabetic mechanisms

Zipora Tietel, Devanesan Arul Ananth, Thilagar Sivasudha · AIMS Agriculture and Food · 2024

Application of Response Surface Methodology for the Extraction of Phytochemicals from Upcycled Kale (Brassica oleracea var. acephala)

Harichandana Valisakkagari, H. P. Vasantha Rupasinghe · Nutraceuticals · 2025

Showing 2 of 3 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

23

Page views

0

PDF downloads

2

Outbound clicks

3

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.