Skip to content
Research Article Open access CC BY 4.0

Optimization of Escherichia coli Bacteriophages Production

V. D. Hari Narayana Kola, Koigoora Srikanth, Shaik Muzammil Pasha, Y. Sudhan Goutham, Chand Pasha

Journal of Advances in Microbiology · pp. 28–37 · Published 4 Apr 2023

10.9734/jamb/2023/v23i4718

Abstract

Aims: Phage therapy may be the best alternative to antibiotics in poultry. There is a need for optimization of phage production conditions to produce more Escherichia coli specific phages for poultry applications. Methodology: An E. coli λ bacteriophage isolated in our laboratory was used for optimization studies. An optimization of the E. coli λ bacteriophage production process was carried out by conventional methods to identify the factors, and the Thaguchi process was used for the optimization of conditions for increased bacteriophage production. With four factors each at three levels L9, experimental runs were carried out. Results: In the conventional method, 8 factors were studied and 4 selected for statistical optimization by Taguchi. At optimized conditions of modified nutrient broth with 1% glycerol, temperature 37oC, 100 rpm for 12 hours of phage multiplication, an improvement of the final titer was achieved from a control 2x109  to optimized 5x1012 Plauque formation Units (PFU)/mL. Conclusion: Phage production is dependent on bacterial growth and bacteriophage multiplication in bacteria. In such a complicated system, the Thaguchi method will be a convenient method of phage production optimization.

Bacteriophage E. coli phage therapy λ Phage Taguchi

References (37)

  1. 1 Molecular cloning: A laboratory manual [DOI]
  2. 2 World Health Organization Global Estimates and Regional Comparisons of the Burden of Foodborne Disease in 2010 [DOI]
  3. 3 Design and Analysis of Experiments [DOI]
  4. 4 Biofilms in the Food Industry: Health Aspects and Control Methods [DOI]
  5. 5 Design of Experiments Using the Taguchi Approach: 16 Steps to Product and Process Improvement [DOI]
  6. 6 Bacteriophage Applications for Food Production and Processing [DOI]
  7. 7 Escherichia coli in Europe: An Overview [DOI]
  8. 8 Phage Choice, Isolation, and Preparation for Phage Therapy [DOI]
  9. 9 Antibiotic resistance in Enterobacteriaceae: mechanisms and clinical implications [DOI]
  10. 10 Survival and growth characteristics of Escherichia coli associated with hemorrhagic colitis [DOI]
  11. 11 Bacteriophages and their application in food safety [DOI]
  12. 12 Use of Lytic Bacteriophage for Control of Experimental Escherichia coli Septicemia and Meningitis in Chickens and Calves [DOI]
  13. 13 Bacteriophage Latent-Period Evolution as a Response to Resource Availability [DOI]
  14. 14 Bacteriophage therapy to combat bacterial infections in poultry [DOI]
  15. 15 The Use of Bacteriophages in the Poultry Industry [DOI]
  16. 16 The Journal of General and Applied Microbiology [DOI]
  17. 17 Phages and Immunomodulation [DOI]
  18. 18 Control of Listeria monocytogenes growth in a ready-to-eat poultry product using a bacteriophage [DOI]
  19. 19 Modeling tailed bacteriophage adsorption: Insight into mechanisms [DOI]
  20. 20 An Optimized Bacteriophage Cocktail Can Effectively Control Salmonella in vitro and in Galleria mellonella [DOI]
  21. 21 Isolation, characterization and application of bacteriophage PSDA-2 against Salmonella Typhimurium in chilled mutton [DOI]
  22. 22 Optimization of Salmonella bacteriophage microencapsulation in alginate-caseinate formulation using vibrational nozzle technique [DOI]
  23. 23 Prevention of Escherichia coli infection in broiler chickens with a bacteriophage aerosol spray [DOI]
  24. 24 Don’t Shut the Stable Door after the Phage Has Bolted—The Importance of Bacteriophage Inactivation in Food Environments [DOI]
  25. 25 Optimization of fermentation parameters in phage production using response surface methodology [DOI]
  26. 26 Determining Optimum Conditions for Lipase-Catalyzed Synthesis of Triethanolamine (TEA)-Based Esterquat Cationic Surfactant by a Taguchi Robust Design Method [DOI]
  27. 27 Oxygen supply in disposable shake-flasks: prediction of oxygen transfer rate, oxygen saturation and maximum cell concentration during aerobic growth [DOI]
  28. 28 Bacteriophage therapy as an alternative biocontrol against emerging multidrug resistant E. coli in broilers [DOI]
  29. 29 Changes in Environmental Conditions Modify Infection Kinetics of Dairy Phages [DOI]
  30. 30 Bacteriophage Esc-A is an efficient therapy for Escherichia coli 3-1 caused diarrhea in chickens [DOI]
  31. 31 Enhancement of asymmetric bioreduction of ethyl 4-chloro acetoacetate by the design of composition of culture medium and reaction conditions [DOI]
  32. 32 Application of Taguchi Experimental Design for the Optimization of Effective Parameters on the Rapeseed Methyl Ester Production [DOI]
  33. 33 Maximizing filamentous phage yield during computer-controlled fermentation [DOI]
  34. 34 A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach [DOI]
  35. 35 Critical Evaluation of Bacteriophage to Prevent and Treat Colibacillosis in Poultry [DOI]
  36. 36 Scale-Up and Bioprocessing of Phages [DOI]
  37. 37 Temperature-dependent changes to host-parasite interactions alter the thermal performance of a bacterial host [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

45

Page views

0

PDF downloads

4

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.