Skip to content
Research Article Open access CC BY 4.0

Optimization, a Potential Production, GC-MS and Characterization of Dark Green Pigment from New Local Isolate Streptomyces nigra Strain GH12

Gehad H. El Sayed, Mohamed Fadel, Mohamed Marzouk

Journal of Advances in Biology & Biotechnology · pp. 11–29 · Published 9 Nov 2023

10.9734/jabb/2023/v26i9653

Abstract

Due to increasing health awareness among consumers and the identification of novel pharmacological properties of diverse natural pigments, the market for natural pigments is currently seeing rapid growth. Streptomyces. nigra GH12, a new Actinomycete isolate identified by 16S rRNA used for dark green pigment production. Manipulations of the factors affecting pigment production are the most effective way to have maximum yield of pigment. Starch 2.5% (w/v), ammonium nitrate 0.2% (w/v), dibasic sodium phosphate 0.1% (w/v), inoculums size 6% (v/v),  initial pH level of 8, 150 rpm rotation speed, 37 ºC temperature, and 9 days of incubation  were found the optimum growth conditions for the highest green  pigment production. The extraction of green pigment was performed efficiently with ethanol solvent. Results showed no effect on the pigment content at 40, 50 and 60°C. On heating pigment extract at 70, 80, 90 and 100oC for 60 min, retention of pigment remained as high as 97.4, 93, 86 and 79% of the total pigment density , respectively, even with continuous treatment at 100°C for more 60 min. The results indicated that the pigment extracts exhibited their greatest stabilities at high pH values, i.e. 8.0, 8.5, 9.0 and 10.0, during the entire holding time. The pigment was characterized by full UV-visible spectroscopic screening, where the potential absorption maximum of the target green pigment extract was recorded in the range of 300 ̶ 360 nm, with a λmax at 340 nm. Moreover, the chemical composition diversity of the pigment extract was further investigated by GC/MS analysis that revealed the presence of 57 metabolites in their silylated form.

Actinomycetes green pigment culture conditions extraction UV and GC/MS characterization

References (38)

  1. 1 The neighbor-joining method: a new method for reconstructing phylogenetic trees. [DOI]
  2. 2 MEGA11: Molecular Evolutionary Genetics Analysis Version 11 [DOI]
  3. 3 MUSCLE: a multiple sequence alignment method with reduced time and space complexity [DOI]
  4. 4 Bioactive Microbial Metabolites [DOI]
  5. 5 Fungal and Bacterial Pigments: Secondary Metabolites with Wide Applications [DOI]
  6. 6 Microbial Pigments in the Food Industry—Challenges and the Way Forward [DOI]
  7. 7 Microbial pigments as natural color sources: current trends and future perspectives [DOI]
  8. 8 Biologically active peptides: Processes for their generation, purification and identification and applications as natural additives in the food and pharmaceutical industries [DOI]
  9. 9 Solid-state fermentation for the production of Monascus pigments from jackfruit seed [DOI]
  10. 10 Marine Streptomyces as a novel source of bioactive substances [DOI]
  11. 11 Sources, stability, encapsulation and application of natural pigments in foods [DOI]
  12. 12 Biosurfactant: Production and Application [DOI]
  13. 13 Red pigment production by Penicillium purpurogenum GH2 is influenced by pH and temperature [DOI]
  14. 14 Natural Pigments of Microbial Origin [DOI]
  15. 15 Production of red pigment by submerged culture of Paecilomyces sinclairii [DOI]
  16. 16 Biotechnological advances for improving natural pigment production: a state-of-the-art review [DOI]
  17. 17 Natural Red Pigment Production by Monascus Purpureus in Submerged Fermentation Systems Using a Food Industry Waste: Brewer’s Spent Grain [DOI]
  18. 18 Regulatory and metabolic network of rhamnolipid biosynthesis: Traditional and advanced engineering towards biotechnological production [DOI]
  19. 19 Streptomyces Secondary Metabolites [DOI]
  20. 20 Jackfruit Seed - A Novel Substrate for the Production of Monascus Pigments through Solid-State Fermentation
  21. 21 Evaluation of Antimicrobial, Enzyme Inhibitory, Antioxidant and Cytotoxic Activities of Partially Purified Volatile Metabolites of Marine Streptomyces sp.S2A [DOI]
  22. 22 Antimicrobial activity of textile fabrics dyed with prodigiosin pigment extracted from marine Serratia rubidaea RAM_Alex bacteria [DOI]
  23. 23 Streptomyces nigra sp. nov. Is a Novel Actinobacterium Isolated From Mangrove Soil and Exerts a Potent Antitumor Activity in Vitro [DOI]
  24. 24 Bacterial Pigments: The Bioactivities and as an Alternative for Therapeutic Applications [DOI]
  25. 25 Streptomyces-Derived Bioactive Pigments: Ecofriendly Source of Bioactive Compounds [DOI]
  26. 26 Optimization of fermentation conditions for red pigment production from Penicillium sp. under submerged cultivation [DOI]
  27. 27 Assessment of the Dyeing Properties of the Pigments Produced by Talaromyces spp. [DOI]
  28. 28 Some Factors Affecting the Production of Carotenoids by Rhodotorula glutinis var. glutinis [DOI]
  29. 29 A comprehensive study of eco-friendly natural pigment and its applications [DOI]
  30. 30 Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, Exiguobacterium sp. PMA [DOI]
  31. 31 Profiling of red pigment produced by Streptomyces sp. JAR6 and its bioactivity [DOI]
  32. 32 Microbial pigments: Learning from Himalayan perspective to industrial applications [DOI]
  33. 33 Monascorubin production by Penicillium minioluteum ED24 in a solid-state fermentation using sesame seed cake as substrate [DOI]
  34. 34 Natural Food Pigments and Colorants [DOI]
  35. 35 Factors Affecting Red Pigment Production by Local Fungal Isolate of Penicillium sp. [DOI]
  36. 36 Optimization of apple pomace based medium and fermentation conditions for pigment production by Sarcina sp.
  37. 37 Extracellular Production And Characterization Of Red Pigment From Penicillium Purpurogenum Bks9 [DOI]
  38. 38 Production of Red Pigment from Fungal Isolate DMMS-1 [DOI]

Cited by 12

Streptomyces zaomycetitus strain GH90: a source of violet pigment with metabolic profiling and potential application in textile: in vitro supported by in silico studies and molecular docking

Gehad H. El Sayed, Mohamed Fadel, Mohamed Marzouk · BMC Microbiology · 2025

Exploring pigment-producing Streptomyces as an alternative source to synthetic pigments: diversity, biosynthesis, and biotechnological applications. A review

Richard Asah-Asante, Lushi Tang, Xiaowei Gong · World Journal of Microbiology and Biotechnology · 2025

Improving natural red pigment production by Streptomyces phaeolivaceus strain GH27 for functionalization of textiles with in silico ADME prediction

Gehad H. El Sayed, Mohamed Fadel, Rasha Fouad · BMC Microbiology · 2025

Liquid chromatography/tandem mass spectrometric characterization of Rhodotorula mucilaginosa GMM natural pigment and its synergistic antitumor effect with γ-radiation: in-vitro and in-vivo study

Ghada S. Ibrahim, Amal I. Hassan, Manal G. Mahmoud · Journal of The Arab Society for Medical Research · 2024

Functionalization of textile using streptomyces erythrogriseus GH80 brown bioactive pigment with in silico studies

Gehad H. El Sayed, Mohamed Fadel, Rasha Fouad · BMC Microbiology · 2025

Showing 5 of 12 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

4

Page views

0

PDF downloads

0

Outbound clicks

12

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.