In vivo Antithrombotic Activity of Ethanolic Extract from Ocimum basilicum L.

Abstract

Aims: In this study, the antithrombotic activity of ethanolic extract from Ocimum basilicum L. was investigated on mice. The active component of Ocimum basilicum L. was extracted in 40% ethanol by Soxhlet extractor. Place and Duration of Study: Department of Pharmacology, Faculty of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang, China, between February, 2009 and July, 2009. Methodology: The crude extract was gavaged to mice in different concentration (100mg/ml, 200 mg/ml, and 400 mg/ml), for 21 days, Salvia Tablets were used as a positive control. On the final day, 50 mg/kg carrageenan was injected intraperitoneally to induce blood coagulation. Fibrin degradation products (FDPs) were assayed. Results: Results showed that the content of mice FDPs significantly（P<0. 05）increased in the 200 mg/ml dose group and 400mg/ml dose group compared to the control group. This was dose-dependent. Conclusion: The ethanolic extract of Ocimum basilicum L can activate the fibrinolytic system so as to stimulate Plasminogen enzyme into fibrinolytic enzyme to show antithrombotic activity.