Effect of Clomiphene Citrate on the Hormonal Profile of Adult Female Wistar Rats with Regular Estrous Cycle

Abstract

This study investigates the effect of Clomiphene citrate (CC) on the Hormonal profile of female Adult Wistar rats with regular estrous cycle. A total of thirty six (36) healthy female Wistar rats were reared and screened for the purpose of this study. The animals were divided into 4 groups consisting of 4 rats each with regular estrous cycle and were treated as follows; Group 1 served as control while Groups 2, 3 and 4 were treated with 1 mg/kg, 2mg/kg and 5 mg/kg body weight of CC respectively, for 11 consecutive days. Body weight was obtained at commencement and every other day of the experiment using digital weighing scale. There was no statistically significant (p>0.05) difference in body weight of rats with regular estrous cycle at day 1, 3, 5 and 7 compared to control. However, there was a statistically significant (p<0.05) difference in body weight across the group on day 9 and 11. Progesterone was significantly (p<0.05) higher in the 1mg as well as 5mg CC treated groups with a significant decrease in follicle stimulating hormone (FSH) value across treatments. CC could improve the sex hormone changes in women due to its anti-estrogenic properties, which is consistent with our results hence, the significant changes observed in the reproductive hormone levels.