Skip to content
Research Article Open access CC BY 4.0

Occurrence of Enteric Pathogens on Fresh Produce Grown on Irrigated Soils

G. O. Abakpa, V. J. Umoh, J. B. Ameh, S. E. Yakubu, J. K. P. Kwaga, A. M. Ibekwe

Microbiology Research Journal International · pp. 13–23 · Published 8 Dec 2014

10.9734/BMRJ/2015/13910

Abstract

Aims: To assess the potential health risks of fresh produce grown on irrigated soils treated with manure in Kano State, a large produce region in Nigeria. Methodology: Fresh produce irrigated with wastewater on manure treated soils were assessed for the prevalence, serotype distribution and toxigenicity of Escherichia coli O157, Salmonella spp and Vibrio cholerae in a large produce region in Nigeria. A total of 230 samples obtained from five designated produce locations were examined using selective isolation method with prior enrichment. Fresh produce comprised carrots, cabbage, cucumber, lettuce, tomatoes, spinach, green peas and spring onions. Suspect isolates were identified and characterized by conventional biochemical methods and Microbact 24E (Oxoid, UK) kit. Confirmed isolates were serotyped and E. coli O157 and Vibrio cholerae O1 were assayed for their toxigenic potentials using the Reverse Passive Latex Agglutination kit. The enterotoxigenicity of Salmonella spp was determined by detection of stn gene using polymerase chain reaction (PCR) techniques. Results: Results obtained showed that overall, Salmonella spp and Escherichia coli O157 had the highest prevalence of 17.0% and 10.9%, respectively. Both were most commonly detected from fresh produce. The serotypes of Salmonella detected include Salmonella typhi (51.3%), Salmonella paratyhpi (20.5%) and Salmonella typhimurium (28.2%); strains of Vibrio cholerae O1 detected include Vibrio cholerae O1 of the Ogawa, Inaba and the Hikojima serotypes. Conclusion: The use of untreated irrigation water in vegetable production represents a significant route of transmission of diarrheal pathogen to humans and hence represents a public health risk. We recommend proper and adequate wastewater treatment before use.

Enteric pathogens fresh produce contamination irrigation water

References (18)

  1. 1 Effects of metals on fish behavior: a review [DOI]
  2. 2 Bacteria in biology, biotechnology, and medicine
  3. 3 Cholera epidemiology in Nigeria: an overview. [DOI]
  4. 4 Improving food safety through a one health approach: workshop summary.
  5. 5 Multidrug Resistance and Plasmid Patterns of Escherichia coli O157 and Other E. coli Isolated from Diarrhoeal Stools and Surface Waters from Some Selected Sources in Zaria, Nigeria [DOI]
  6. 6 Soil Organic Matter Dynamics in the Subhumid Agroecosystems of the Ethiopian Highlands [DOI]
  7. 7 Diversity, Frequency, and Persistence ofEscherichia coliO157 Strains from Range Cattle Environments [DOI]
  8. 8 Occurrence of pathogens on vegetables harvested from soils irrigated with contaminated streams [DOI]
  9. 9 Escherichia coli contamination and health aspects of soil and tomatoes (Solanum lycopersicum L.) subsurface drip irrigated with on-site treated domestic wastewater [DOI]
  10. 10 Toxigenic Vibrio cholerae O1 in Water and Seafood, Haiti [DOI]
  11. 11 Outbreak of Salmonella serotype Saintpaul infections associated with eating alfalfa sprouts - United States, 2009.
  12. 12 EMERGING FOODBORNE PATHOGENS AND PROBLEMS: EXPANDING PREVENTION EFFORTS BEFORE SLAUGHTER OR HARVEST
  13. 13 Bacterial indicators of pollution of the Douala lagoon, Cameroon: public health implications.
  14. 14 Role of Salmonella enterotoxin in overall virulence of the organism [DOI]
  15. 15 Fractionation and mobility of cadmium and zinc in urban vegetable gardens of Kano, Northern Nigeria [DOI]
  16. 16 Epidemiology and antibiotic resistance of Salmonella enterica Serovar Kentucky isolates from Tunisia: The new emergent multi-drug resistant serotype [DOI]
  17. 17 Incidences Of Endophytic Human Pathogens In Fresh Produce
  18. 18 Global Burden of Disease [DOI]

Cited by 4

Molecular characterization of Salmonella spp. isolates from river and dam water, irrigated vegetables, livestock, and poultry manures in Jordan

Yaser H. Tarazi, Abdallah F. Al Dwekat, Z. Ismail · Veterinary World · 2021

Showing 1 of 4 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

73

Page views

0

PDF downloads

29

Outbound clicks

4

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.