Skip to content
Research Article Open access CC BY 4.0

Multiple Shoots Induction from Indigenous Nigerian Pumpkin (Cucurbita pepo L.)

Olawole O. Obembe, Oluwadurotimi S. Aworunse, Oluwakemi A. Bello, Abosede O. Ani

Annual Research & Review in Biology · pp. 1–10 · Published 1 Oct 2017

10.9734/ARRB/2017/35756

Abstract

Aim: The aim of this study was to develop a protocol for in vitro regeneration of a Nigerian indigenous pumpkin (C. pepo L.) via seedling-derived cotelydon, cotyledonary node and hypocotyl explants. Study Design: A combination of 0.00, 1.00, 2.00 and 3.00 mgl-1 of 6-Benzylaminopurine (BAP) and 0.00 or 0.05 mgl-1 of 2,4-Dichlorophenoxy acetic acid (2,4-D) for each explant type were set up in three replicates making a total of 36 culture vessels in the entire experimental set up. Five explants were cultured per combination. Place and Duration of Study: The work was conducted in the Plant Tissue Culture Laboratory of the Department of Biological Sciences, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria, between January 2017 and July 2017. Methodology: Hypocotyl, cotyledonary node and cotyledon explants derived from 4-week old-seedlings were cultured on MS medium fortified with 0.00, 1.00, 2.00 and 3.00 mgl-1 of BAP in combination with 0.00 or 0.05 mgl-1 of 2,4-D and investigated for callus, shoot and root induction. Results: A combination of 1.00 mgl-1 BAP with 0.05 mgl-1 2,4-D was optimum for callus induction from hypocotyl and cotyledonary node explants, while for cotyledon explants, 2.00 mgl-1 BAP in combination with 0.05 mgl-1 2,4-D was preferred. Cotyledonary node explants and cotyledonary node explant-derived callus responded with multiple shoots (4.50±0.042 and 4.07±0.067 shoots per explant, respectively) on full strength Murashige and Skoog (MS) medium (control) devoid of Plant Growth Regulators (PGRs). When the different explant types were cultured on MS media amended with the different concentrations of BAP in combination with 2,4-D, neither shoot nor root induction was observed. All the explants initiated roots when cultured on full strength PGR-free medium. Only cotyledon explant-derived callus formed roots (2.30±0.56cm) on PGR-free MS medium. Conclusion: Regeneration of indigenous vegetables, such as Cucurbita pepo is achievable through hypocotyl, cotyledonary node and cotyledon explants with or without PGRs.

BAP 2,4-D cotyledonary node cotyledon hypocotyl

References (22)

  1. 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
  2. 2 Review of Cucurbita pepo (Pumpkin) its Phytochemistry and Pharmacology [DOI]
  3. 3 Rapid in vitro propagation of medicinally important Ceropegia candelabrum [DOI]
  4. 4 Meristem, shoot tip and bud cultures
  5. 5 Shoot production in squash (Cucurbita pepo) by in vitro organogenesis [DOI]
  6. 6 Indirect Organogenesis in Summer Squash (Cucurbita pepo L.)
  7. 7 Biodiesel Production via Esterification of Free Fatty Acids from Cucurbita pepo L. Seed Oil: Kinetic Studies
  8. 8 Historical records, origins, and development of the edible cultivar groups ofCucurbita pepo (Cucurbitaceae) [DOI]
  9. 9 Shoot regeneration and the relationship between organogenic capacity and endogenous hormonal contents in pumpkin [DOI]
  10. 10 In vitro organogenesis in watermelon cotyledons [DOI]
  11. 11 'In vitro' Micropropagation through Cotyledonary Node Culture of Castor Bean ('Ricinus communis' L.)
  12. 12 Growth, yield and antioxidant profile of pumpkin (Cucúrbita pepo L.) leafy vegetable as affected by NPK compound fertilizer [DOI]
  13. 13 Chemical evaluation of seeded fruit biomass of oil pumpkin (Cucurbita pepo L. var. Styriaca) [DOI]
  14. 14 Micropropagation of Kalanchoe tubiflora (Harvey) Hamet [DOI]
  15. 15 Relationships between hormonal contents and the organogenic response in Pinus pinea cotyledons [DOI]
  16. 16 Somatic Embryogenesis and Regeneration from Cotyledon Explants of Six Squash Cultivars [DOI]
  17. 17 Towards conservation of Nigerian medicinal plants [DOI]
  18. 18 In Vitro Propagation of Pumpkin and Ash Gourd through Nodal Segments [DOI]
  19. 19 Basic Aspects of Differentiation and Plant Regeneration from Cell and Tissue Cultures [DOI]
  20. 20 Rapid In Vitro Micro Propagation Of Chick Pea (Cicer Arietinum L.) From Shoot Tip And Cotyledonary Node Explants [DOI]
  21. 21 Plant Biotechnology on a flight: Is Africa on board? [DOI]
  22. 22 Use of somatic embryogenesis as a vehicle for cotton transformation [DOI]

Cited by 6

Amino Acid Profile, Functional Properties and In-vitro Antioxidant Capacity of Cucurbita maxima and Cucurbita mixta Fruit Pulps and Seeds

Aleem Waheed Oyeleke, David Timilehin Oluwajuyitan, Olusola Matthew Oluwamukomi, Ndigwe Victor Enujiugha · European Journal of Nutrition & Food Safety · 2019

Protocol for ex vitro and in vitro micropropagation of Cucurbita moschata and C. ecuadorensis, native to Peru and Ecuador, of nutritional and medicinal importance

G. E. Delgado-Paredes, C. Rojas-Idrogo, Cecilia Vásquez-Díaz · Scientia Agropecuaria · 2023

Chromosome stability of in vitro propagated Cucurbita cultivars

Buse Dursun, A. A. Uncuoglu, Y. Aydin · 2019

Effect of low BAP Levels on Multiple Shoots Induction in Indigenous Nigerian Pumpkin (Cucurbita pepo Linn.)

Oluwadurotimi Samuel Aworunse, Regina Voke Omasoro, Bukola Soneye · Journal of Physics, Conference Series · 2019

In vitro Multiple Shoots Formation in Wild Lettuce(Launaea taraxacifolia) (Willd.) Amin ex C. Jeffrey

O. Obembe, O. Bello, Oluwadurotimi S. Aworunse · 2017

In vitro regeneration of hybrid squash (Cucurbita pepo L.) cv. First Runner from cotyledonary node explants

K. Sarker, A. Chowdhury, F. Monshi · Fundamental and Applied Agriculture · 2022

Related research

Article metrics

Real usage data collected on this platform.

72

Page views

0

PDF downloads

31

Outbound clicks

6

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.