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Research Article Open access CC BY 4.0

Morphological and Molecular Characterization of Colletotrichum gloeosporioides Causing Mango Anthracnose

M. Kaviyarasi, A. Kamalakannan, L. Rajendran, S. Rajesh, M. Kavino, K. R. Swarna Lakshmi, J. Shajith Basha

International Journal of Plant & Soil Science · pp. 837–844 · Published 7 Jul 2022

10.9734/ijpss/2022/v34i2031230

Abstract

Mango (Mangifera indica), a fruit with high nutritional value is widely regarded as a most palatable fruit crop which is affected by a number of biological constraints mainly diseases. Anthracnose caused by Colletotrichum gloeosporioides belongs to order Melanconiales is the important post harvest disease which restricts the marketing of mango. In the present study, thirteen isolates obtained from various places of Tamil Nadu were collected and isolated by tissue segment approach and purified. The isolates were confirmed phenotypically using morphological characters. Molecular investigation like PCR assay using universal primers ITS1 and ITS4 produced amplicon size of 560bp. The isolates were also identified using genus specific  and species specific primers which resulted in the amplicon size of 280bp and 380bp. As a result, The use of morphological and molecular approaches to characterise Mango anthracnose will be useful in identifying and managing the disease.

Mangifera indica Colletotrichum gloeosporioides molecular characterization

Cited by 4

First Report of Occurrence of Colletotrichum siamense Causing Grape Anthracnose in Punjab, India

Harmeen Kaur, Amrinder Kaur, Anita Arora · National academy science letters · 2026

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