Morphofunctional Changes in Basophilic Cells of the Adenohypophysis during Post-resuscitation Disease

Abstract

In the post-resuscitation period through the I-II-III-IV-V - stages and long - term periods (1 and 3 months) of post – resuscitation disease, the morpho functional activity of β-and d-basophil cells of the adenohypophysis was studied in white male rats with a weight of 180-220 g, who suffered a 10-minute stop of systemic circulation (compression of the vascular bundle of the heart by the method of V. G. Korpachev). False-operated animals served as a control. Morphological, morphometric, histochemical, and cytophotometric studies of the state of both β - and d - basophil cells of the adenohypophysis were performed. We used image analysis using a leys microscope with an electronic micro-nozzle attached to an Intel computer, and the content of the glycoprotein was studied using a cytophotometer. In the postoperative period during the phase I identified in the background of gipergidratace cytoplasmic β - and d - basophilic cells of the anterior pituitary, in the period of II-III stage disease gipergidratace nuclei β - and d - basophilic cells of the anterior pituitary revealed an increase in the excretion of glycoprotein in blood, i.e., the observed compensatory-adaptive reactions. Starting from stage IV and in remote periods of post-resuscitation disease, compensatory and restorative processes were observed against the background of hyperhydration of the nuclei.