Molecular Detection of Yellow Mosaic Virus Infecting Black Gram and Green Gram in Coimbatore District

Abstract

Yellow mosaic virus (YMV) is indeed one of the most destructive diseases of mungbean (Vigna radiata (l.) wilczek) and urdbean (Vigna mungo l. Hepper). Mungbean Yellow Mosaic Virus infecting green gram and black gram cause great yield loss.The virus belongs to the genus begomovirus of the family geminiviridae transmitted by white fly. Infected samples were collected and scored according to disease scale. Diseased samples were collected from infected plants from the field of Coimbatore district and percent disease index was calculated. The symptoms of yellow mosaic virus ranges from small yellow spots which develop as mosaic yellow patterns. Later the disease leads to complete yellowing of the leaf samples. Percent Disease Index impart the severity of virus in black gram and green gram field.The DNA was isolated from both black gram and green gram samples through CTAB method. Both the quality and quantity of the DNA was evaluated through gel electrophoresis and nanodrop reading and found to be appreciably good. The DNA was molecularly confirmed with universal primer which show amplification around 1200 bp through Polymerase Chain Reaction (PCR). Polymerase chain reaction of Yellow Mosaic Virus infecting black gram and green gram samples using MYMV-CP-f/MYMV-cp-r primers of DNA A component were amplified and the expected product of size 1000 bp from the obtained infected samples.