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Research Article Open access CC BY 4.0

Genetic Identification of Species of Bats that Act as Reservoirs or Hosts for Viral Diseases

Pedro Carnieli Jr, Karin Corrêa Scheffer, Willian Oliveira Fahl, Jonas Yoshitaka de Oliveira Lima, Rafael de Novaes Oliveira, Juliana Galera Castilho, Keila Iamamoto, Carla Isabel Macedo, Paulo Eduardo Brandão, Helena Beatriz de Carvalho Ruthner Batista

Annual Research & Review in Biology · pp. 1–9 · Published 18 Dec 2015

10.9734/ARRB/2016/23295

Abstract

Introduction: Viruses have been identified as the main etiologic agents of both zoonoses and emerging infectious diseases (EIDs) and various species of wild fauna can be involved in the maintenance of these diseases. The very wide variety of bats, together with their ability to adapt to different environments and fly long distances, means that these animals are currently one of the main reservoirs for zoonoses and EIDs. For these reasons the correct identification of different bat species is essential. Aims: This paper describes the genetic identification of 56 samples isolated from different bat species. Methodology: Sequencing and phylogenetic analysis of the mitochondrial DNA cytochrome b (mtDNA cyt-b) gene.  Results: Four families (Molossidae, Vespertilionidae, Noctilionidae and Phyllostomidae), twelve genera and nineteen different species of bats were identified, and the Basic Local Alignment Search Tool (BLAST) was used to confirm species identity.  The phylogenetic tree constructed revealed two main clusters (1 and 2), both consist in two subclusters. Conclusions: Our results were concordant with those obtained by morphometric identification and genetic identification carried out by other authors, showing that the method described here can be used as an effective alternative to, or in combination with, morphometric identification of bats.

Bats virus wild reservoirs cytochrome b.

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Cited by 6

Identification of Different Species of Mammalians Involved in Zoonoses as Reservoirs or Hosts by Sequencing of the Mitochondrial DNA Cytochrome B Gene

Pedro Carnieli Jr, Juliana Galera Castilho, Rafael de Novaes Oliveira, Paulo Eduardo Brandão, Helena Beatriz de Carvalho Ruthner Batista · Annual Research & Review in Biology · 2016

Revising the paradigm: Are bats really pathogen reservoirs or do they possess an efficient immune system?

M. Weinberg, Y. Yovel · iScience · 2022

Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies

D. A. Caraballo, M. E. Montani, Leila M Martínez · PLoS ONE · 2020

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