Molecular Detection of Transgenes in Improved Varieties of Cowpea
Kingsley Onuh, Karderam Bukar Dikwa, Victoria Moltong Dan, Mohammed Sani Abdulsalami, Ugochukwu Okechukwu Ozojiofor, Enimie Endurance Oaikhena, Kenneth Onuh, Ebipade Kereakede, Asabe Danjuma
International Journal of Biochemistry Research & Review · pp. 1–9 · Published 29 Aug 2022
10.9734/ijbcrr/2022/v31i830340Abstract
Cowpea is one of the most consumed food in Africa. Recombinant DNA technology has been used to insert foreign genes into plants genome via cassettes there by creating a new generation of plants with desired traits. Such plants possess improved seed quality, yield and resistance to pests and pathogens. The aim of this study is to screen for transgene in Cowpeas sold in Nigerian markets using molecular analysis. Five Improved varieties of Cowpea were obtained from IITA while local varieties were purchased from Terminus Market in Jos. All samples were screened for the presence of transgene via DNA extraction and PCR. Results shows the presence of transgene in MKT1, IT89KD and IT99KD, CAMV, NOS and VIP3BA respectively and none was found in local varieties.
References (23)
- 1 Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. [DOI]
- 2 Current perspectives on genetically modified crops and detection methods [DOI]
- 3 Transgenic cowpeas (Vigna unguiculata L. Walp) expressing Bacillus thuringiensis Vip3Ba protein are protected against the Maruca pod borer (Maruca vitrata) [DOI]
- 4 Bacillus thuringiensis Cry1Ia10 and Vip3Aa protein interactions and their toxicity in Spodoptera spp. (Lepidoptera) [DOI]
- 5 Recombinase Polymerase Amplification (RPA) of CaMV-35S Promoter and nos Terminator for Rapid Detection of Genetically Modified Crops [DOI]
- 6 New multiplex PCR methods for rapid screening of genetically modified organisms in foods [DOI]
- 7 PCR detection of genetically modified maize and soy in mildly and highly processed foods [DOI]
- 8 Assessment of the Antimicrobial Activity and the Entomocidal Potential of Bacillus thuringiensis Isolates from Algeria [DOI]
- 9 Characterization of Bacillus thuringiensis isolates by their insecticidal activity and their production of Cry and Vip3 proteins [DOI]
- 10 Assessing the genetic diversity of cowpea [Vigna unguiculata (L.) Walp.] accessions from Sudan using simple sequence repeat (SSR) markers [DOI]
- 11 Activity of vegetative insecticidal proteins Vip3Aa58 and Vip3Aa59 of Bacillus thuringiensis against lepidopteran pests [DOI]
- 12 A simple and accurate PCR method for detection of genetically modified rice [DOI]
- 13 Techniques for detecting genetically modified crops and products [DOI]
- 14 Detection approaches for genetically modified organisms in foods [DOI]
- 15 Impact of Climate Change on Agricultural Production in the Sahel – Part 2. Case Study for Groundnut and Cowpea in Niger [DOI]
- 16 Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method [DOI]
- 17 Development of insect-resistant transgenic cotton with chimeric TVip3A* accumulating in chloroplasts [DOI]
- 18 Influence of cocoa components on the PCR detection of soy lecithin DNA [DOI]
- 19 Screening food products for the presence of CaMV 35S promoter and NOS 3′ terminator [DOI]
- 20 Development of a CTAB buffer-based automated gDNA extraction method for the surveillance of GMO in seed [DOI]
- 21 The analysis of food samples for the presence of Genetically Modified Organisms - User Manual [DOI]
- 22 Detection of CaMV-35S Promoter and NOS Terminator in Genetically Modified Tomato Seed in Iraqi Markets
- 23 Molecular detection of genetically modified organisms in seeds from local Iraqi markets [DOI]
Cited by 1
Ugochukwu Ozojiofor · Journal of Current Biomedical Research · 2023
Related research
- Effects of Fermentation and Extrusion on the Proximate and Organoleptic Properties of Cowpea-plantain Flour Blends — shares topic coverage
- Assessing the Microbiological Level and the Incidence of Water-soaking on the Proximate Composition of two Cultivars of Cowpea (Vigna unguiculata L.) Grains Grown in Côte d’Ivoire — shares topic coverage
- Effect of Neem Oil in Combination with Propiconazole and Organic Amendments on Anthracnose (Colletotrichum lindemuthianum) of Cowpea (Vigna unguiculata L.) — shares topic coverage
- Investigation in Minerals Nutrients from Powders Deriving with Leaflets of Moringa oleifera L. and Beans of Vigna unguiculata W. for Fortification of the Flour Processed from New Shoots of Borassus aethiopum M. — shares topic coverage
- Efficiency of Triple Bagging System and Lippia multiflora Leaves for the Vitamin Quality Preservation of Cowpea Seeds (Vigna unguiculata L. Walp) — shares topic coverage
Article metrics
Real usage data collected on this platform.
25
Page views
0
PDF downloads
3
Outbound clicks
1
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.