Skip to content
Research Article Open access CC BY 4.0

Diversity of Rotavirus VP7 and VP4 Genotypes Associated with Severe Childhood Diarrhea in North West Cameroon: Detection of Unusual Strains G1P[6], G2P[6], G2P[8] and G3P[6]

Florence A. Mbuh, Susan Damanka, Gorge E. Armah, Sunday A. Omilabu, Aliyu A. Ahmad, Jarlath U. Umoh

Microbiology Research Journal International · pp. 1–11 · Published 9 Jun 2015

10.9734/BMRJ/2015/18071

Abstract

Aims: To determine rotavirus genotypes and distribution among children 0 - 5 years old with severe acute diarrhea in the North West Region (NWR) of Cameroon. Study Design: Cross sectional. Place and Duration of Study: Hospitals and health centers in the NWR served as sample collection centers between January and December 2004. Methodology: Fifty-six Enzyme Immunoassay (EIA) and Polyacrylamide gel electrophoresis (PAGE) - positive rotavirus stool specimens were analyzed for VP7 and VP4 genotypes by reverse transcriptase polymerase chain reaction (RT-PCR). Results: A total of 51 (91.1%) samples genotyped as G and P types while the remaining five samples (8.9%) were partially characterized. Four VP7 genotypes (G1, G2, G3 and G9) and two VP4 genotypes, P[6] and P[8] were detected. The predominant G and P types were; G3 (34%) and P[8] (41.1%). Genotypes G1-G3 accounted for 85.8% of isolates while G9 represented 3.6% of isolates. Genotype P[6] represented 30.4% of all VP4 genotypes and was the most widespread strain occurring in all age groups. Twelve single G and P-type combinations were identified. Genotype G1P[8] (19.6%) predominated, followed by G3P[6] (17.9%). Unusual strains detected were G1P[6], G2P[6], G2P[8] and G3P[6] accounting for 32% of cases. Mixed infections were detected from 15 (27%) isolates comprising G1/G3P[6], G2/G9P[6], G2P[6]/P[8] and G1/G3/G8 P[6]/P[8]. Twenty isolates (35.7%) had unusual genotype / electropherotypes combinations. All G9 strains, mixed G genotypes and strains with atypical electrpherotypes occurred in children hospitalized with severe gastroenteritis. Conclusion: There is high incidence of unusual rotavirus strains circulating in the NWR of Cameroon that could have an impact on rotavirus vaccine performance. Future studies will investigate post vaccine prevalence and characterization of non-typeable strains by other methods.

Diarrhea gastroenteritis rotavirus genotypes Cameroon Central Africa

References (20)

  1. 1 Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens [DOI]
  2. 2 Identification of group A rotavirus gene 4 types by polymerase chain reaction [DOI]
  3. 3 Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine [DOI]
  4. 4 Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels [DOI]
  5. 5 Serotype Diversity and Reassortment between Human and Animal Rotavirus Strains: Implications for Rotavirus Vaccine Programs [DOI]
  6. 6 Global Mortality Associated with Rotavirus Disease among Children in 2004 [DOI]
  7. 7 Systematic review of regional and temporal trends in global rotavirus strain diversity in the pre rotavirus vaccine era: Insights for understanding the impact of rotavirus vaccination programs [DOI]
  8. 8 Identification of an avian group A rotavirus containing a novel VP4 gene with a close relationship to those of mammalian rotaviruses [DOI]
  9. 9 Rotavirus G and P types in children with acute diarrhea in Blantyre, Malawi, from 1997 to 1998: Predominance of novel P[6]G8 Strains [DOI]
  10. 10 Rotavirus VP4 and VP7 Genotypes Circulating in Cameroon: Identification of Unusual Types [DOI]
  11. 11 Epidemiologic and Genotypic Characteristics of Rotavirus Strains Detected in Children Less Than 5 Years of Age With Gastroenteritis Treated at 3 Pediatric Hospitals in Zimbabwe During 2008–2011 [DOI]
  12. 12 Molecular epidemiology of rotavirus in black infants in South Africa [DOI]
  13. 13 Characterization of incompletely typed rotavirus strains from Guinea-Bissau: identification of G8 and G9 types and a high frequency of mixed infections [DOI]
  14. 14 Rotavirus typing methods and algorithms [DOI]
  15. 15 Rotavirus genotypes associated with childhood severe acute diarrhoea in southern Ghana: a cross-sectional study [DOI]
  16. 16 Preparing for the Scale-up of Rotavirus Vaccine Introduction in Africa [DOI]
  17. 17 Molecular epidemiology of group A human rotaviruses in North West region, Cameroon. [DOI]
  18. 18 Unusual human rotavirus strains having subgroup I specificity and ?long? RNA electropherotype [DOI]
  19. 19 Inaccurate identification of rotavirus genotype G9 as genotype G3 strains due to primer mismatch [DOI]
  20. 20 The use of repetitive DNA probes in the analysis of natural populations of insects and parasites.

Cited by 1

Prevalence of rotavirus A infection and the detection of type G3P[11] strain in ruminants in Yobe state, Nigeria

John Garba, O. O. Faleke, A. Magaji · Tropical Animal Health and Production · 2020

Related research

Article metrics

Real usage data collected on this platform.

70

Page views

0

PDF downloads

45

Outbound clicks

1

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.