Skip to content
Research Article Open access

Mercuric Chloride Toxicity and Its Influence on Enzymatic Antioxidants – A Study on Zebra Fish Model (Danio rerio)

Jyotsna ., Alok Kumar Madeshia, B. Prabasheela

Current Journal of Applied Science and Technology · pp. 4130–4135 · Published 1 Aug 2014

10.9734/BJAST/2014/10374

Abstract

Enzymatic antioxidant was determined to establish a possible environmental impact on toxic metal in fish Danio rerio. Alterations in the antioxidant cellular systems have often been proposed as biomarkers of pollutant-mediated toxicity. This prompted us to investigate the activities of catalase, superoxide dismutase, Reduce Glutathione in the liver of mercuric chloride treated zebra fish. The fishes were exposed to Mercuric chloride for a period of 96 hours. The LC50 value was obtained by using probit software based on 50% of fish that died at each concentration. Mortality was recorded at 24th, 48th, 72nd and 96th hours. Various concentrations of Mercuric chloride (70µg/ml and 250µg/ml as 1st stage, 20mg/ml, 30mg/ml, 50mg/ml and 60mg/ml as 2nd stage, and 5mg/ml, 2.5mg/ml, 2mg/ml, 1mg/ml and 0.5mg/ml as 3rd stage) were injected into the tanks containing the fishes for 96 hours with a photoperiod of 12hours. After the 96 hours, Liver of the fishes was collected in an Eppendorf and stored at -20ºC for further analysis. In the present study, increase in SOD activity as well as Catalase activity and a decrease in glutathione activity were registered.  

Catalase superoxide dis mutase glutathione mercuric chloride prooxidant antioxidant.

References (18)

  1. 1 Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase [DOI]
  2. 2 Biomarkers of Oxidative Stress and Heavy Metal Levels as Indicators of Environmental Pollution in African Cat Fish (Clarias gariepinus) from Nigeria Ogun River [DOI]
  3. 3 Increasing Rates of Atmospheric Mercury Deposition in Midcontinental North America [DOI]
  4. 4 Biomarkers of oxidative stress: a comparative study of river Yamuna fish Wallago attu (Bl. & Schn.) [DOI]
  5. 5 Mercury toxicity and antioxidants: Part 1: role of glutathione and alpha-lipoic acid in the treatment of mercury toxicity.
  6. 6 Combined effect of zinc and lead on the hepatic superoxide dismutase-catalase system in carp (Cyprinus carpio) [DOI]
  7. 7 Effects of Positive Iron Status at a Cellular Level [DOI]
  8. 8 Damage to plasmid DNA by singlet oxygen and its protection [DOI]
  9. 9 The protective effects of glutathione against methylmercury cytotoxicity [DOI]
  10. 10 Distribution of micro-essential (Fe, Cu, Zn) and toxic (Hg) metals in tissues of two nutritionally distinct hydrothermal shrimps [DOI]
  11. 11 Effects of zinc and cadmium on HgCl2-δ-ALA-D inhibition and Hg levels in tissues of suckling rats [DOI]
  12. 12 Mercury in swedish forest soils and waters — Assessment of critical load [DOI]
  13. 13 Association of GlutathioneS-Transferase Isozyme-Specific Induction and Lipid Peroxidation in Two Inbred Strains of Mice Subjected to Chronic Dietary Iron Overload [DOI]
  14. 14 ANTIOXIDATIVE ENZYMES IN FISH AS BIOCHEMICAL INDICATORS OF AQUATIC POLLUTION
  15. 15 Chelating efficacy of CaNa2 EDTA on nickel-induced toxicity in Cirrhinus mrigala (Ham.) through its effects on glutathione peroxidase, reduced glutathione and lipid peroxidation [DOI]
  16. 16 Pathophysiology and Reactive Oxygen Metabolites [DOI]
  17. 17 Effect of lead nitrate on thyroid function of the Indian palm squirrel,Funambulus pennanti (Wroughton) [DOI]
  18. 18 The role of oxygen free radicals in biology and evolution

Cited by 3

Mercuric chloride‐induced oxidative stress, genotoxicity, haematological changes and histopathological alterations in fish Channa punctatus ( B loch, 1793)

Sunil P. Trivedi, Shefalee Singh, Abha Trivedi · Journal of Fish Biology · 2022

Mercuric Chloride induced Oxidative Stress, Genotoxicity, Haematological Changes and Histopathological Alterations in Fish Channa Punctatus (Bloch, 1793).

Sunil P. Trivedi, Shefalee Singh, Abha Trivedi · 2021

Showing 2 of 3 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

54

Page views

0

PDF downloads

16

Outbound clicks

3

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.