Skip to content
Research Article Open access CC BY 4.0

Manganese Inhibits Indomethacin-Induced Hepatorenal Oxidative Stress in Wistar Rats

Tijani Stephanie Abiola, Olori Ogaraya David, Farombi Ebenezer Olatunde

International Journal of Biochemistry Research & Review · pp. 79–90 · Published 27 Nov 2020

10.9734/ijbcrr/2020/v29i930227

Abstract

Aim: Manganese (Mn) is an essential trace element in many cellular processes. However, there is dearth of literature on its influence on indomethacin-induced hepatorenal damage. Therefore, this study was conducted to investigate the effect of manganese on indomethacin-induced hepatorenal damage in rats. Methods: Rats were divided into four groups of eight rats consisting of control group, indomethacin (IND) alone (20 mg/kg), Mn alone (10 mg/kg) and co-treated group that were treated orally for 14 consecutive days. Twenty four hours after treatment, under pentobarbital anesthesia, blood was collected and liver was excised to prepare homogenate and histology staining. Liver and kidney function tests aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glutamine dehydrogenase (GLDH), sorbitol dehydrogenase (SDH), glucose-6-phosphate dehydrogenase (G6PD), bilirubin (BIL), urea, creatinine, cholesterol (CHOL), triglycerides (TG), low and high density lipoprotein (LDL and HDL), electrolytes and oxidative stress superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and lipid peroxidation (LPO) biomarkers were assessed. Results: The results showed that indomethacin caused hepatorenal damage in rats manifested with increase in serum hepatic and renal function biomarkers. But co-administration of IND with Mn significantly (p < 0.05) decreased the level of hepatorenal biomarkers. Additionally, co-administration of IND with Mn improved the antioxidant status with concomitant reduction of LPO and restored the integrity of the liver and kidney histologically. Conclusion: The results of this study emphasize that co-administration of IND with Mn to rats alleviated IND-induced hepatorenal toxicities and oxidative stress in rats.

Hepatorenal indomethacin manganese lipid peroxidation rats.

References (24)

  1. 1 The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase [DOI]
  2. 2 Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method [DOI]
  3. 3 The Role of Oxidative Stress and Antioxidants in Liver Diseases [DOI]
  4. 4 Oxidative stress as a crucial factor in liver diseases [DOI]
  5. 5 Comparative study of the efficacy and safety of paracetamol, ibuprofen, and indomethacin in closure of patent ductus arteriosus in preterm neonates [DOI]
  6. 6 Hepatoprotective effects of methanol extract of Carissa opaca leaves on CCl4-induced damage in rat [DOI]
  7. 7 Alteration of saliva and serum concentrations of manganese, copper, zinc, cadmium and lead among career welders [DOI]
  8. 8 Relationships Between Essential Manganese Biology and Manganese Toxicity in Neurological Disease [DOI]
  9. 9 Cholesterol: recapitulation of its active role during liver regeneration [DOI]
  10. 10 Epidemiological survey among workers exposed to manganese: Effects on lung, central nervous system, and some biological indices [DOI]
  11. 11 Effect of manganese supplementation and source on carcass traits, meat quality, and lipid oxidation in broilers1 [DOI]
  12. 12 Manganese accumulation in bone following chronic exposure in rats: Steady-state concentration and half-life in bone [DOI]
  13. 13 Effect of Nigella sativa Linn oil on tramadol-induced hepato- and nephrotoxicity in adult male albino rats [DOI]
  14. 14 Autophagy plays a protective role in Mn-induced toxicity in PC12 cells [DOI]
  15. 15 Occupational manganese exposure, reproductive hormones, and semen quality in male workers: A cross-sectional study [DOI]
  16. 16 Emerging evidence in NSAID pharmacology: important considerations for product selection.
  17. 17 Low doses of multi-walled carbon nanotubes elicit hepatotoxicity in rats with markers of oxidative stress and induction of pro-inflammatory cytokines [DOI]
  18. 18 The effects of the inhaled formaldehyde during the early postnatal period in the hippocampus of rats: A morphological and immunohistochemical study [DOI]
  19. 19 Zinc prevents indomethacin-induced renal damage in rats by ameliorating oxidative stress and mitochondrial dysfunction [DOI]
  20. 20 Orexin receptor expression is increased during mancozeb-induced feeding impairments and neurodegenerative events in a marine fish [DOI]
  21. 21 The rodenticidal effect of indomethacin: pathogenesis and pathology
  22. 22 Hepatoprotective and antioxidative effect of ethanolic leaf extract of Langenaria breviflora (bitter gourd) on indomethacin-ulcerated rats [DOI]
  23. 23 ABC gene-ranking for prediction of drug-induced cholestasis in rats [DOI]
  24. 24 Multisystem toxicity of indomethacin: Effects on kidney, liver and intestine in the rat [DOI]

Cited by 5

THE PROTECTIVE EFFECT OF TURMERIC RHIZOMES EXTRACT FROM HEPATOTOXICITY AND NEPHROTOXICITY INDUCED BY INDOMETHACIN

GHADA HASSAN, NAHLA IBRAHIM, AKIL HAJJOUZ · Asian Journal of Pharmaceutical and Clinical Research · 2026

Anti-inflammatory potential of black cumin seed oil and its nanoemulsion formulation against lipopolysaccharide-induced liver injury in mice

Hesham Haffez, Rehab Hosni, Shady A. Swidan · Asian Pacific Journal of Tropical Biomedicine · 2024

Showing 2 of 5 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

11

Page views

0

PDF downloads

0

Outbound clicks

5

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.